Separation and identification of oligonucleotides by hydrophilic interaction liquid chromatography (HILIC)-inductively coupled plasma mass spectrometry (ICPMS)

被引：53

作者：

Easter, Renee N. ^{[1
]}

Kroening, Karolin K. ^{[1
]}

Caruso, Joseph A. ^{[1
]}

Limbach, Patrick A. ^{[2
]}

机构：

[1] Univ Cincinnati, Dept Chem, Agilent Technol Metall Ctr Amer, Cincinnati, OH 45221 USA

[2] Univ Cincinnati, Dept Chem, Rieveschl Labs Mass Spectrometry, Cincinnati, OH 45221 USA

来源：

ANALYST | 2010年 / 135卷 / 10期

基金：

美国国家卫生研究院;

关键词：

NUCLEIC-ACIDS; PROTEIN-PHOSPHORYLATION; SPECIATION ANALYSIS; SELENIUM; PHASE; PHOSPHORUS; CISPLATIN; SAMPLES; HPLC;

D O I：

10.1039/c0an00399a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the separation and detection of oligonucleotides utilizing hydrophilic interaction liquid chromatography (HILIC) with inductively coupled plasma mass spectrometry (ICPMS) is described. Polythymidylic acids of various lengths (10, 15, 20 and 30 nucleotides) were separated under gradient HILIC conditions. Selective detection of oligonucleotides was possible through monitoring m/z 47, corresponding to (PO+)-P-31-O-16, using ICPMS. Oxygen was used as a reaction gas in the collision/reaction cell to produce PO+ by reacting with phosphorus in the gas phase, thereby effectively eliminating the interferences for phosphorus normally seen at m/z 31. Limits of detections (LODs) were determined to be 1.69 pmol, 1.21 pmol, 1.0 pmol and 0.55 pmol loaded on column for the 10, 15, 20 and 30 mer, respectively.

引用

页码：2560 / 2565

页数：6

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