Combination of separation and spectroscopic analytical techniques: application to compositional analysis of a minor citrus species

被引：16

作者：

Costa, Rosaria ^{[1
]}

Salvo, Andrea ^{[1
,2
]}

Rotondo, Archimede ^{[1
]}

Bartolomeo, Giovanni ^{[1
]}

Pellizzeri, Vito ^{[1
]}

Saija, Emanuele ^{[1
]}

Arrigo, Sara ^{[1
]}

Interdonato, Monica ^{[4
]}

Trozzi, Alessandra ^{[3
]}

Dugo, Giacomo ^{[1
,2
]}

机构：

[1] Univ Messina, Dept Biomed & Dent Sci & Morphofunct Imaging, Messina, Italy

[2] Univ Messina, Science4Life, Spin Off Co, Messina, Italy

[3] Univ Messina, Dipartimento Sci Chim Biol Farmaceut & Ambientali, Messina, Italy

[4] Univ Messina, Dipartimento Sci Matemat Informat Sci Fis & Sci T, Messina, Italy

来源：

NATURAL PRODUCT RESEARCH | 2018年 / 32卷 / 21期

关键词：

Citrus limetta Risso; enantiomer; juice; NMR; polyphenols; heartcutting; LINEAR RETENTION INDEXES; ESSENTIAL OIL; MASS-SPECTROMETRY; NMR-SPECTROSCOPY; LIMETTA RISSO; GC-MS; JUICE; IDENTIFICATION; VOLATILE; CHROMATOGRAPHY;

D O I：

10.1080/14786419.2018.1428597

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The composition of juice and essential oil of Citrus limetta Risso was investigated. Multidimensional and enantio-gas chromatography were used for the elucidation of the volatile profile and the assessment of enantiomeric distribution. Predominant compounds were linalyl acetate (13.06 g/100 g), beta-pinene (6.79 g/100 g), myrcene (1.40 g/100 g) and sabinene (1.05 g/100 g). Through a beta-cyclodextrin column eight chiral pairs were separated, equally distributed between laevorotatory and dextrorotatory enantiomers. Liquid chromatography with triple quadrupole ESI-MS and PDA detection showed the presence of flavonoids and phenolic compounds (791.34 +/- 36.83 and 32.97 +/- 1.92 mg L-1, respectively) in the juice. Sugars (i.e. glucose 202.23 +/- 26.81 and fructose 146.73 +/- 3.17 mM, respectively), aminoacids (i.e. proline 29.40 +/- 3.78 and GABA 8.90 +/- 0.95 mM) and organic acids (i.e. citrate 4.68 +/- 0.81 mM) were determined in juice by means of H-1 NMR spectroscopy. [GRAPHICS] .

引用

页码：2596 / 2602

页数：7