Production of bacterial cellulose by Gluconacetobacter sp RKY5 isolated from persimmon vinegar

被引:49
作者
Kim, Soo-Yeon [2 ]
Kim, Jin-Nam [2 ]
Wee, Young-Jung [1 ]
Park, Don-Hee [1 ]
Ryu, Hwa-Won [1 ]
机构
[1] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Dept Mat Chem & Biochem Engn, Kwangju 500757, South Korea
关键词
bacterial cellulose; fermentation; Gluconacetobacter; optimization; persimmon vinegar;
D O I
10.1385/ABAB:131:1:705
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The optimum fermentation medium for the production of bacterial cellulose (BC) by a newly isolated Gluconacetobacter sp. RKY5 was investigated. The optimized medium composition for cellulose production was determined to be 15 g/L glycerol, 8 g/L yeast extract, 3 g/L K2HPO4, and 3 g/L acetic acid. Under these optimized culture medium, Gluconacetobacter sp. RKY5 produced 5.63 g/L of BC after 144 h of shaken culture, although 4.59 g/L of BC was produced after 144 h of static culture. The amount of BC produced by Gluconacetobacter sp. RKY5 was more than 2 times in the optimized medium found in this study than in a standard Hestrin and Shramm medium, which was generally used for the cultivation of BC-producing organisms.
引用
收藏
页码:705 / 715
页数:11
相关论文
共 15 条
[1]   Structure of Acetobacter cellulose composites in the hydrated state [J].
Astley, OM ;
Chanliaud, E ;
Donald, AM ;
Gidley, MJ .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2001, 29 (03) :193-202
[2]  
Brown AJ, 1886, J CHEM SOC, V49, P432, DOI [DOI 10.1039/CT8864900432, 10.1039/CT8864900432]
[3]  
Buchanan R. E., 1984, Bergey's Manual of Determinative Bacteriology
[4]   Aggregation of ribbons in bacterial cellulose induced by high pressure incubation [J].
Hult, EL ;
Yamanaka, S ;
Ishihara, M ;
Sugiyama, J .
CARBOHYDRATE POLYMERS, 2003, 53 (01) :9-14
[5]   Production and application of microbial cellulose [J].
Jonas, R ;
Farah, LF .
POLYMER DEGRADATION AND STABILITY, 1998, 59 (1-3) :101-106
[6]   A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp sucrofermentans [J].
Matsuoka, M ;
Tsuchida, T ;
Matsushita, K ;
Adachi, O ;
Yoshinaga, F .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (04) :575-579
[7]   Production of bacterial cellulose by Gluconacetobacter hansenii PJK isolated from rotten apple [J].
Park, JK ;
Park, YH ;
Jung, JY .
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2003, 8 (02) :83-88
[8]  
Ryu H. W., 2004, Korea patent application, Patent No. [10-2004-0104790, 1020040104790]
[9]   SYNTHESIS OF CELLULOSE BY ACETOBACTER-XYLINUM .1. MICROMETHOD FOR THE DETERMINATION OF CELLULOSES [J].
SCHRAMM, M ;
HESTRIN, S .
BIOCHEMICAL JOURNAL, 1954, 56 (01) :163-166
[10]  
Son C, 2002, J MICROBIOL BIOTECHN, V12, P722