Imbalance between endothelial progenitors cells and microparticles in HIV-infected patients naive for antiretroviral therapy

被引:45
作者
da Silva, Erika F. R. [1 ]
Fonseca, Francisco A. H. [2 ]
Franca, Carolina N. [2 ]
Ferreira, Paulo R. A. [1 ]
Izar, Maria C. O. [2 ]
Salomao, Reinaldo [1 ]
Camargo, Luciano M. [2 ]
Tenore, Simone B. [1 ]
Lewi, David S. [1 ]
机构
[1] Univ Fed Sao Paulo, Div Infect Dis, Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Cardiovasc Div, Lipids Atherosclerosis & Vasc Biol Sect, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
endothelial progenitor cells; endothelial-derived microparticles; HIV infection; inflammation; platelet-derived microparticles; LOW-DENSITY-LIPOPROTEIN; DISEASE RISK-FACTORS; C-REACTIVE PROTEIN; MEMBRANE MICROPARTICLES; CARDIOVASCULAR-DISEASE; ATHEROSCLEROSIS; DIFFERENTIATION; ANGIOGENESIS; MOBILIZATION; ACTIVATION;
D O I
10.1097/QAD.0b013e32834980f4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: Cardiovascular events have been reported among HIV-infected patients following antiretroviral therapy. However, the role of HIV itself in determining vascular damage is less described. Chronic inflammatory state might impair some regulatory endothelium properties leading to its activation, apoptosis or erosion. Objectives: To evaluate the balance between endothelial progenitor cells and micro-particles in HIV-infected antiretroviral drug-naive patients. Design: A case-control study comparing HIV-infected patients (n = 35) with sex-matched and age-matched healthy controls (n = 33). Methods: Endothelial progenitor cells populations expressing CD34(+), CD133(+) and KDR+ were quantified by flow cytometry. Endothelial-derived microparticles, expressing CD51(+), and platelet-derived microparticles, expressing CD31(+)/CD42(+), were also measured. Endothelial function was estimated by flow-mediated dilation. Results: Lower percentages of endothelial progenitor cells (CD34(+)/KDR+) were observed in HIV-infected individuals compared with controls (0.02 vs. 0.09%, P = 0.045). In addition, endothelial microparticles concentration was higher in HIV-infected individuals (1963 vs. 436 microparticles/ml platelet-poor plasma, P = 0.003), with similar number of platelet-derived microparticles among groups. Furthermore, flow-mediated dilation was lower among HIV-infected individuals compared with controls [mean (SEM): 10 (1) and 16% (2), respectively; P = 0.03]. Conclusion: Our findings suggest an imbalance between endothelial progenitor cells mobilization and endothelial apoptosis. The alteration in the turnover of endothelial cells may contribute to cardiovascular events in HIV-infected patients. (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins
引用
收藏
页码:1595 / 1601
页数:7
相关论文
共 48 条
[1]   Microparticles: Key Protagonists in Cardiovascular Disorders [J].
Amabile, Nicolas ;
Rautou, Pierre-Emmanuel ;
Tedgui, Alain ;
Boulanger, Chantal M. .
SEMINARS IN THROMBOSIS AND HEMOSTASIS, 2010, 36 (08) :907-916
[2]   Cross-sectional study of endothelial function in HIV-infected patients in Brazil [J].
Andrade, Ana Cristina O. ;
Ladeia, Ana Marice ;
Netto, Eduardo M. ;
Mascarenhas, Amanda ;
Cotter, Bruno ;
Benson, Constance A. ;
Badaro, Roberto .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 2008, 24 (01) :27-33
[3]   Endothelial microparticles and platelet and leukocyte activation in patients with the metabolic syndrome [J].
Arteaga, Roque B. ;
Chirinos, Julio A. ;
Soriano, Andres O. ;
Jy, Wenche ;
Horstman, Lawrence ;
Jimenez, Joaquin J. ;
Mendez, Armando ;
Ferreira, Alexandre ;
de Marchena, Eduardo ;
Ahn, Yeon S. .
AMERICAN JOURNAL OF CARDIOLOGY, 2006, 98 (01) :70-74
[4]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[5]   Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization [J].
Asahara, T ;
Masuda, H ;
Takahashi, T ;
Kalka, C ;
Pastore, C ;
Silver, M ;
Kearne, M ;
Magner, M ;
Isner, JM .
CIRCULATION RESEARCH, 1999, 85 (03) :221-228
[6]   High-Density Lipoprotein Particles and Markers of Inflammation and Thrombotic Activity in Patients with Untreated HIV Infection [J].
Baker, Jason ;
Ayenew, Woubeshet ;
Quick, Harrison ;
Hullsiek, Katherine Huppler ;
Tracy, Russell ;
Henry, Keith ;
Duprez, Daniel ;
Neaton, James D. .
JOURNAL OF INFECTIOUS DISEASES, 2010, 201 (02) :285-292
[7]   Endothelial cell activation, injury, damage and dysfunction: separate entities or mutual terms? [J].
Blann, AD .
BLOOD COAGULATION & FIBRINOLYSIS, 2000, 11 (07) :623-630
[8]   Circulating microparticles - A potential prognostic marker for atherosclerotic vascular disease [J].
Boulanger, Chantal M. ;
Amabile, Nicolas ;
Tedgui, Alain .
HYPERTENSION, 2006, 48 (02) :180-186
[9]   Early Increase in Autoantibodies Against Human Oxidized Low-Density Lipoprotein in Hypertensive Patients After Blood Pressure Control [J].
Brandao, Sergio A. ;
Izar, Maria C. ;
Fischer, Simone M. ;
Santos, Andreza O. ;
Monteiro, Carlos M. ;
Povoa, Rui M. ;
Helfenstein, Tatiana ;
Carvalho, Antonio C. ;
Monteiro, Andrea M. ;
Ramos, Eduardo ;
Gidlund, Magnus ;
Figueiredo Neto, Antonio M. ;
Fonseca, Francisco A. .
AMERICAN JOURNAL OF HYPERTENSION, 2010, 23 (02) :208-214
[10]   Cell-derived microparticles in haemostasis and vascular medicine [J].
Burnier, Laurent ;
Fontana, Pierre ;
Kwak, Brenda R. ;
Angelillo-Scherrer, Anne .
THROMBOSIS AND HAEMOSTASIS, 2009, 101 (03) :439-451