S100A11 influences the invasion and migration of lung cancer cells by the Notch signaling pathway

Objective: To explore the expression of S100A11 in lung cancer tissues, its effects on the invasion and migration abilities of lung cancer cells and the relevant mechanism. Methods: the expression of S100A11 was detected in lung cancer and normal lung tissues by Western blotting; lentivirus silencing S100A11, GFP fluorescence and Western blotting were used to detect the LV3-S100A11 silencing efficiency and efficacy; the effect of S100A11 expression on invasion of lung cancer cells was detected by Transwell invasion assay; the effect of S100A11 expression on migration of lung cancer cells was detected by wound scratch assay; the expression of the proteins Notch1, NICD1 and Hes1 was detected by Western blotting; and the effects of silencing S100A11 on size and volume of lung cancer were detected in the subcutaneous tumor formation experiment in nude mice. Results: the expression of S100A11 increased significantly compared with that in normal lung tissues, and the high expression of S100A11 was related to the pathological type, differentiation degree and metastasis of local lymph nodes in lung cancer; LV3-S100A11 lentivirus could effectively inhibit the expression of S100A11; silencing S100A11 inhibited the invasion and migration abilities of the lung cancer A549 cells; silencing S100A11 could down-regulate the expression of Notch1, NICD1 and Hes1; and the tumor volume and weight of tumor-bearing mice decreased significantly in the LV3-S100A11 group as compared to the LV3-NC group. Conclusion: S100A11 influences the invasion and migration abilities of lung cancer cells by the Notch signaling pathway.