Cellular uptake and mutagenic potential of metal oxide nanoparticles in bacterial cells

被引:204
作者
Kumar, Ashutosh [1 ]
Pandey, Alok K. [1 ]
Singh, Shashi S. [2 ]
Shanker, Rishi [1 ]
Dhawan, Alok [1 ]
机构
[1] Indian Inst Toxicol Res, CSIR, Nanomat Toxicol Grp, Lucknow 226001, Uttar Pradesh, India
[2] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India
关键词
Nanoparticle uptake; Bacteria; S; typhimurium; Flow cytometry; ZnO and TiO2 nanoparticles; Ames mutagenicity test; ULTRAFINE TIO2 PARTICLES; ZNO NANOPARTICLES; TOXICITY; CYTOTOXICITY; GENOTOXICITY; SURFACE; IMPACT; TESTS;
D O I
10.1016/j.chemosphere.2011.01.025
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Extensive production and consumption of nanomaterials such as ZnO and TiO2 has increased their release and disposal into the environment. The accumulation of nanoparticles (NPs) in ecosystem is likely to pose threat to non-specific targets such as bacteria. The present study explored the effect of ZnO and TiO2 NPs in a model bacterium, Salmonella typhimurium. The uptake of ZnO and TiO2 bare NPs in nano range without agglomeration was observed in S. typhimurium. TEM analysis demonstrated the internalization and uniform distribution of NPs inside the cells. Flow cytometry data also demonstrates that both ZnO and TiO2 NPs were significantly internalized in the S. typhimurium cells in a concentration dependent manner. A significant increase in uptake was observed in the S. typhimurium treated even with 8 and 80 ng ml(-1) of ZnO and TiO2 NPs with S9 after 60 min, possibly the formation of micelles or protein coat facilitated entry of NPs. These NPs exhibited weak mutagenic potential in S. typhimurium strains TA98, TA1537 and Escherichia coli (WP2uvrA) of Ames test underscoring the possible carcinogenic potential similar to certain mutagenic chemicals. Our study reiterates the need for re-evaluating environmental toxicity of ZnO and TiO2 NPs presumably considered safe in environment. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1124 / 1132
页数:9
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