Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection

被引：108
作者：
Bell, Jason B. [1 ]
Podetz-Pedersen, Kelly M. [1 ]
Aronovich, Elena L. [1 ]
Belur, Lalitha R. [1 ]
McIvor, R. Scott [1 ,2 ]
Hackett, Perry B. [1 ]
机构：
[1] Univ Minnesota, Inst Human Genet, Beckman Ctr Transposon Res, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[2] Discovery Genom Inc, Minneapolis, MN 55413 USA
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 12期
关键词：
D O I：
10.1038/nprot.2007.471
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system combines the advantages of viruses and naked DNA molecules for gene therapy purposes; however, efficacious delivery of DNA molecules to animal tissues can still be problematic. Here we describe the hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse. The procedure involves rapid, high-pressure injection of a DNA solution into the tail vein. The overall procedure takes <1 h although the delivery into one mouse requires only a few seconds. Successful injections result in expression of the transgene in 5-40% of hepatocytes 1 d after injection. Several weeks after injection, transgene expression stabilizes at similar to 1% of the level at 24 h, presumably owing to integration of the transposons into chromosomes.
引用
收藏
页码：3153 / 3165
页数：13
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