Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

被引：15
|
作者：
Lee, Jeeyeon [1 ,2 ]
Jeong, Jiyeon [1 ]
Lee, Heeyoung [2 ]
Ha, Jimyeong [1 ,2 ]
Kim, Sejeong [1 ,2 ]
Choi, Yukyung [1 ,2 ]
Oh, Hyemin [1 ,2 ]
Seo, Kunho [3 ]
Yoon, Yohan [1 ,2 ]
Lee, Soomin [2 ]
机构：
[1] Sookmyung Womens Univ, Dept Food & Nutr, Seoul 04310, South Korea
[2] Sookmyung Womens Univ, Risk Anal Res Ctr, Seoul 04310, South Korea
[3] Konkuk Univ, Dept Vet Med, Seoul 05029, South Korea
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2017年 / 14卷 / 11期
关键词：
Campylobacter; poultry; antibiotic susceptibility; Rep-PCR; cdt toxin; CYTOLETHAL DISTENDING TOXIN; MULTIPLEX PCR ASSAY; ANTIMICROBIAL RESISTANCE; SPP; CONTAMINATION; CHICKEN CARCASSES; ESCHERICHIA-COLI; RISK-FACTORS; JEJUNI; PREVALENCE; IDENTIFICATION;
D O I：
10.3390/ijerph14111400
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in Campylobacter isolates from poultry. Chicken (n = 152) and duck (n = 154) samples were collected from 18 wet markets in Korea. Campylobacter spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLab(TM) system and were tested for the presence of cytolethal distending toxin (cdt) genes. Campylobacter spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of Campylobacter contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three cdt genes (cdtA, cdtB, and cdtC) were present in 71.1% of Campylobacter isolates. These results indicate that Campylobacter contamination should be decreased to prevent and treat Campylobacter foodborne illness.
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页数：11
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