A dorsal model for cutaneous vesicant injury by 2-chloroethyl ethyl sulfide using C57BL/6 mice

被引：12

作者：

Isidore, Myriane A.

Castagna, Michael P.

Steele, Keith E.

Gordon, Richard K.

Nambiar, Madhusoodana P. ^{[1
]}

机构：

[1] Walter Reed Army Inst Res, Dept Biochem Pharmacol, Div Biochem, Silver Spring, MD 20910 USA

[2] Walter Reed Army Inst Res, Dept Immunohistochem, Div Pathol, Silver Spring, MD USA

[3] Uniformed Serv Univ Hlth Sci, Dept Med, Bethesda, MD 20814 USA

来源：

CUTANEOUS AND OCULAR TOXICOLOGY | 2007年 / 26卷 / 03期

关键词：

2-chloroethyl ethyl sulfide; blistering agents; cutaneous injury; chemical warfare agent; mice; sulfur mustard; skin; vesicants;

D O I：

10.1080/15569520701521914

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

To evaluate stem cell-derived therapeutics for cutaneous vesicant injuries, we developed a dorsal exposure model using C57BL/6 black mice and half-mustard, 2-chloroethyl ethyl sulfide ( CEES). The dorsal side of a mouse was exposed to 1-5 mu l of CEES for 10 minutes and then decontaminated. The data demonstrate that 3 mu l of CEES induced edema and erythema that peaked 24 h post exposure. Histopathology showed a central area of deep injury characterized by severe necrosis of epidermis and dermis. The C57BL/6 is a unique model that can be used to unravel the molecular mechanism of injury, identify the effects of black skin pigmentation, and evaluate the efficacy of stem cell therapeutics for cutaneous vesicant exposure.

引用

页码：265 / 276

页数：12

共 25 条

[1] AGENTS OF CHEMICAL WARFARE - SULFUR MUSTARD [J].