Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases

被引:144
作者
Hajighasemi, Mahbod [1 ]
Nocek, Boguslaw P. [2 ]
Tchigvintsev, Anatoli [1 ]
Brown, Greg [1 ]
Flick, Robert [1 ]
Xu, Xiaohui [1 ]
Cui, Hong [1 ]
Hai, Tran [3 ]
Joachimiak, Andrzej [2 ]
Golyshin, Peter N.
Savchenko, Alexei [1 ]
Edwards, Elizabeth A. [1 ]
Yakunin, Alexander F. [1 ]
机构
[1] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada
[2] Argonne Natl Lab, Biosci Div, 9700 S Cass Ave, Argonne, IL 60439 USA
[3] Univ Bangor, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales
基金
美国能源部; 加拿大自然科学与工程研究理事会;
关键词
LOW-MOLECULAR-WEIGHT; POLY(L-LACTIDE) FILM; DEGRADING ENZYME; LACTIC-ACID; DEGRADATION; PURIFICATION; DEPOLYMERASE; HYDROLYSIS; POLYMERS; PLASTICS;
D O I
10.1021/acs.biomac.6b00223
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polylactic acid (PLA) is a biodegradable polyester derived from renewable resources, which is a leading candidate for the replacement of traditional petroleum-based polymers. Since the global production of PLA is quickly growing, there is..an urgent need for the development of efficient recycling technologies, which will produce lactic acid instead of CO2 as the final product. After screening 90 purified microbial alpha/beta-hydrolases, we, identified 'hydrolytic activity against emulsified PLA in two uncharacterized proteins, ABO2449 from Alcanivorax borkumnsis and RPA1511 from Rhodopseudomonas palustris. Both enzymes were also active against emulsified polycaprolactone and other polyesters as well as against soluble alpha-naphthyl and p-nitrophenyl monoesters. In addition, both ABO2449 and RPA1511 catalyzed complete or extensive hydrolysis of solid PLA with the production of lactic acid monomers, dimers, and larger oligomers as products. The crystal structure of RPA1511 was determined at 2.2 A resolution and revealed a classical alpha/beta-hydrolase fold with a wide-operractive site containing a molecule of polyethylene glycol bound near the catalytic triad Ser114-His270-Asp242. Site-directed mutagenesis of both proteins demonstrated that the catalytic triad residues are important for the hydrolysis of both monoester and polyester substrates. We also identified several residues in RPA1511 (Gln172, Leu212, Met215, Trp218, and Leu220) and AB02449 (Phe38 and Leu152), winch were not essential for activity against soluble monoesters but were found to be critical for the hydrolysis of PIA. Out results indicate that microbial carboxyl esterases can efficiently hydrblyze various polyesters malting them attractive biocatalysts for plastics,depolymerization and recycling.
引用
收藏
页码:2027 / 2039
页数:13
相关论文
共 73 条
[1]   PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].
Adams, Paul D. ;
Afonine, Pavel V. ;
Bunkoczi, Gabor ;
Chen, Vincent B. ;
Davis, Ian W. ;
Echols, Nathaniel ;
Headd, Jeffrey J. ;
Hung, Li-Wei ;
Kapral, Gary J. ;
Grosse-Kunstleve, Ralf W. ;
McCoy, Airlie J. ;
Moriarty, Nigel W. ;
Oeffner, Robert ;
Read, Randy J. ;
Richardson, David C. ;
Richardson, Jane S. ;
Terwilliger, Thomas C. ;
Zwart, Peter H. .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :213-221
[2]  
Akutsu Y, 1998, APPL ENVIRON MICROB, V64, P62
[3]   Cloning and sequencing of a poly(DL-lactic acid) depolymerase gene from Paenibacillus amylolyticus strain TB-13 and its functional expression in Escherichia coli [J].
Akutsu-Shigeno, Y ;
Teeraphatpornchai, T ;
Teamtisong, K ;
Nomura, N ;
Uchiyama, H ;
Nakahara, T ;
Nakajima-Kambe, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :2498-2504
[4]  
Albertsson A. C., 2000, CONTROLLED RING OPEN, V2000, P39
[5]   Sustainability of bio-based plastics: general comparative analysis and recommendations for improvement [J].
Alvarez-Chavez, Clara Rosalia ;
Edwards, Sally ;
Moure-Eraso, Rafael ;
Geiser, Kenneth .
JOURNAL OF CLEANER PRODUCTION, 2012, 23 (01) :47-56
[6]  
[Anonymous], 2015, PLAST THE FACTS 2015
[7]   Bacterial lipolytic enzymes: classification and properties [J].
Arpigny, JL ;
Jaeger, KE .
BIOCHEMICAL JOURNAL, 1999, 343 :177-183
[8]   A RAPID COLORIMETRIC ASSAY FOR SERUM LACTIC DEHYDROGENASE [J].
BABSON, AL ;
PHILLIPS, GE .
CLINICA CHIMICA ACTA, 1965, 12 (02) :210-&
[9]   Electrostatics of nanosystems: Application to microtubules and the ribosome [J].
Baker, NA ;
Sept, D ;
Joseph, S ;
Holst, MJ ;
McCammon, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (18) :10037-10041
[10]  
Cai H, 1996, J POLYM SCI POL PHYS, V34, P2701, DOI 10.1002/(SICI)1099-0488(19961130)34:16<2701::AID-POLB2>3.0.CO