Isolation and identification of phenolic compounds from Gynura divaricata leaves

被引:71
作者
Wan, Chunpeng [1 ]
Yu, Yanying [2 ]
Zhou, Shouran [3 ]
Tian, Shuge [4 ]
Cao, Shuwen [1 ,2 ]
机构
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
[2] Nanchang Univ, Dept Chem, Nanchang 330031, Jiangxi, Peoples R China
[3] Jiangxi Univ Tradit Chinese Med, Nanchang 330006, Jiangxi, Peoples R China
[4] Xinjiang Key Lab Famous Prescript & Sci Formulas, Urumqi 830011, Xinjiang, Peoples R China
关键词
Gynura divaricata DC; HPLC-DAD-ESI-MS; phenolic constituents; SCAVENGING ACTIVITY; GLYCOSIDES; FLAVONOIDS; ACIDS; L;
D O I
10.4103/0973-1296.80666
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Background: Phenolic constituents were the principle bioactivity compounds exist in Gynura divaricata, little phenolic compounds were reported from the plant previously. Materials and Methods: 60 ethanol extract from the leaves of Gynura divaricata were isolated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Additionally, a high-performance liquid chromatography-diode array detector-electrospray ionization-mass (HPLC-DAD-ESI-MS) analytical method was developed to identify some minor constituents in the n-butanol fraction of the ethanol extract of Gynura divaricata. Results: Six flavonols and one Dicaffeoylquinic acid were isolated from the leaves of Gynura divaricata, and these compounds were identified as follows: quercetin (1), kaempferol (2), kaempferol-3-O--D-glucopyranoside (3), quercetin-3-O-rutinoside (4), kaempferol-3,7-di-O--D-glucopyranoside (5), kaempferol-3-O-rutinoside-7-O--D-glucopyranoside (6), and 3,5-dicaffeoylquinic acid (7). A total of 13 compounds, including 9 flavonol glycosides and 4 phenolic acids, were tentatively identified by comparing their retention time (RT), UV, and MS spectrum values with those that had been identified and the published data. Conclusion: This was the first time to use the HPLC-DAD-ESI-MS method to identify the phytochemicals of the genera Gynura. Moreover, compounds (6) and (7) have been isolated for the first time from the genus Gynura.
引用
收藏
页码:101 / 108
页数:8
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