Antimicrobial resistance and molecular characterisation of E. coli from poultry in Eastern India

被引:9
作者
Kaushik, Purushottam [1 ]
Anjay [1 ]
Kumari, Savita [1 ]
Dayal, Shanker [2 ]
Kumar, Sunil [1 ]
机构
[1] Bihar Anim Sci Univ, Bihar Vet Coll, Patna 800014, Bihar, India
[2] Indian Council Agr Res, Reg Ctr Eastern Reg, Patna 800014, Bihar, India
关键词
Poultry; E; coli; Anti-microbial resistance; Molecular characterisation; stx(1); stx(2); ENTEROPATHOGENIC ESCHERICHIA-COLI; SALMONELLA SEROVARS; VIRULENCE FACTORS; CHICKEN MEAT; ANTIBIOTICS; ANTIBIOGRAM; PREVALENCE; SEROTYPES; ANIMALS; HUMANS;
D O I
10.12834/VetIt.330.1382.2
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study 252 poultry samples comprised of poultry meat (n = 228) and poultry eggs (n = 24) were screened for the isolation of Escherichia coli (E. coli). A total of 62 E. coli isolates were recovered from poultry meat. The E. coli isolates belonged to different serogroups based on 'O' serotyping of the isolates viz O29 (10.8%), O8 (7.7%), O40 (6.15%), O2 (4.61%), O60 (3.08%), O106 (3.08%), 42 (1.54%), O 87 (1.54%), and 01 serotypes of O1, O7, O30, O45, O59, O66, O105, O1116, O136, O141, O147, O148, O166, and O172. Sixteen (24.62%) of the isolates were UT (untypable) and 6 (9.23 %) were rough types. Molecular characterisation of the isolates was performed, targeting stx(1) and stx(2) virulence gene fragment. Out of 62 E. coli isolates, 10 (16.12%) were carrying virulence gene stx(2), whereas none of the isolate was carrying stx(1) gene. The E. coli isolates showed wide variation in resistance pattern against the antimicrobial agents that we used (9-90%). Among E. coli isolates, maximum resistance was observed against cefuroxime (89.1%) and penicillin (89.4%), followed by ampicillin (80.43%), vancomycin (74.1%), co-trimoxazole (73.1%), cephalothin (60.8%), ceftriaxone (28.2%), tetracycline (17.4%), gentamicin (13%), amikacin (13.04%), ofloxacin (13%), and ciprofloxacin (6.5%). A high degree of susceptibility was observed against amikacin (84.7%) and ciprofloxacin (76%) followed by gentamicin (71.73) and ofloxacin (60.86%). High multiple antibiotic resistances were observed and a total of 34 resistance patterns were identified.
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页码:197 / 204
页数:8
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