Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems

被引：86

作者：

Justesen, Ulrik Stenz ^{[1
]}

Holm, Anette ^{[2
]}

Knudsen, Elisa ^{[1
]}

Andersen, Line Bisgaard ^{[2
]}

Jensen, Thoger Gorm ^{[1
]}

Kemp, Michael ^{[1
]}

Skov, Marianne Nielsine ^{[1
]}

Gahrn-Hansen, Bente ^{[1
]}

Moller, Jens Kjolseth ^{[2
]}

机构：

[1] Odense Univ Hosp, Dept Clin Microbiol, DK-5000 Odense C, Denmark

[2] Vejle Hosp, Dept Clin Microbiol, Vejle, Denmark

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 12期

关键词：

ROUTINE IDENTIFICATION; REVOLUTION; LABORATORIES;

D O I：

10.1128/JCM.05788-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.

引用

页码：4314 / 4318

页数：5

共 19 条

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