Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth

被引：42

作者：

Ahmad, Zulfiqar ^{[1
]}

Laughlin, Thomas F. ^{[1
]}

Kady, Ismail O. ^{[2
]}

机构：

[1] AT Still Univ Hlth Sci, Kirksville Coll Osteopath Med, Dept Biochem, Kirksville, MO 63501 USA

[2] E Tennessee State Univ, Dept Chem, Johnson City, TN 37614 USA

来源：

PLOS ONE | 2015年 / 10卷 / 05期

基金：

美国国家卫生研究院;

关键词：

CATALYTIC SITES; CANCER-CELLS; MYCOBACTERIUM-TUBERCULOSIS; ANTIBACTERIAL ACTIVITY; F1F0-ATP SYNTHASE; MUTANT STRAINS; NIGELLA-SATIVA; RESVERATROL; POLYPHENOLS; F1-ATPASE;

D O I：

10.1371/journal.pone.0127802

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We examined the thymoquinone induced inhibition of purified F-1 or membrane bound F1FO E. coli ATP synthase. Both purified F-1 and membrane bound F1FO were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F1Fo and purified F-1 preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase.

引用

页数：12

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