Capillary electrophoresis of subcellular-sized particles

被引：61

作者：

Radko, SP ^{[1
]}

Chrambach, A ^{[1
]}

机构：

[1] NICHHD, Lab Cellular & Mol Biophys, Macromol Anal Sect, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 722卷 / 1-2期

关键词：

polystyrene-size standards; inorganic particles; organic particles; lipoprotein particles; liposomes; microsomes; viruses;

D O I：

10.1016/S0378-4347(98)00307-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Utilization of capillary electrophoresis (CE) for characterization and analytical separation of submicron- and micron-sized organic and inorganic particles as well as biological vesicles is reviewed. CE has been applied to charged polystyrene size standards, inorganic and organic colloidal particles, lipoprotein particles, liposomes, microsomes and viruses. These particle separations generally occur in a size-dependent manner and provide values of electrophoretic mobility which are in good agreement with those obtained by other electrophoretic techniques. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 10

页数：10

共 53 条

[1] CAPILLARY HYDRODYNAMIC CHROMATOGRAPHY - AN INVESTIGATION INTO OPERATIONAL CHARACTERISTICS [J].

BROUGH, AWJ ;

HILLMAN, DE ;

PERRY, RW .

JOURNAL OF CHROMATOGRAPHY, 1981, 208 (02) :175-182

[2] SIZE SEPARATION OF POLYSTYRENE SULFATE PARTICLES (189 TO 1085 NM RADIUS) IN SOLUTIONS OF METHYL-HYDROXYPROPYL-CELLULOSE OF DIFFERENT CHAIN LENGTHS [J].

BURROUGHS, JA ;

CHRAMBACH, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) :1070-1074

[3]

CHANG HT, 1995, APPL THEOR ELECTROPH, V5, P73

[4] Isolation of subcellular-sized particles separated by electrophoresis in dilute polymer solution, using commercial electrophoresis apparatus with intermittent scanning of fluorescence [J].

Chen, N ;

Chrambach, A .

ELECTROPHORESIS, 1997, 18 (05) :781-783

[5]

Cruzado ID, 1996, J CAPILLARY ELECTROP, V3, P25

[6] AN ANALYSIS OF THE SEPARATION OF SUB-MICRON PARTICLES BY CAPILLARY HYDRODYNAMIC FRACTIONATION (CHDF) [J].

DOSRAMOS, JG ;

SILEBI, CA .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1989, 133 (02) :302-320

[7]

Ducatte GR, 1996, J MICROCOLUMN SEP, V8, P403, DOI 10.1002/(SICI)1520-667X(1996)8:6<403::AID-MCS5>3.3.CO

[8]

2-W

[9]

FOUREST B, 1994, RADIOCHIM ACTA, V66-7, P173

[10] Application of capillary electrophoresis to the characterization of some colloidal particles [J].

Fourest, B ;

Hakem, N ;

Perrone, J ;

Guillaumont, R .

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY-ARTICLES, 1996, 208 (01) :309-318

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