Vitamin K Supplementation Modulates Bone Metabolism and Ultra-Structure of Ovariectomized Mice

被引：13

作者：

Azevedo Rangel, Leticia Batista ^{[1
,2
,3
,4
]}

de Siqueira, Daniel ^{[1
,2
]}

Soares, Olivia do Rosario ^{[1
,2
]}

Santana, Higor Scardini ^{[1
]}

Miguel, Emilio de Castro ^{[1
]}

da Cunha, Maura ^{[5
]}

de Abreu Oliveira, Andre Lacerda ^{[5
]}

Pedrosa, Diego Franca ^{[1
,2
]}

Rangel Resgala, Ludmilla Carvalho ^{[1
,2
,6
]}

Rangel Neto, Helder Azevedo ^{[1
]}

Gomes-Rochette, Neuza Felix ^{[1
]}

Eis, Sergio Ragi ^{[8
]}

Graceli, Jones Bernardes ^{[1
,2
,7
,8
]}

Silva, Ian Victor ^{[1
,2
,3
]}

机构：

[1] Univ Fed Espirito Santo, Dept Morphol, Aging Cell Biol Lab, 1468 Marechal Campos Av Basic I,Room 5, BR-29043090 Vitoria, ES, Brazil

[2] Univ Fed Espirito Santo, RENORBIO, Hlth Sci Ctr, Programa Pos Grad Biotecnol, Vitoria, Brazil

[3] Univ Fed Espirito Santo, Dept Physiol Sci, Hlth Sci Ctr, Programa Pos Grad Bioquim & Farmacol, Vitoria, Brazil

[4] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Pharmaceut Sci, Lab Cellular & Mol Biol Human Canc, Vitoria, Brazil

[5] Univ Estadual N Fluminen, Lab Cell Biol, Biotechnol Ctr, Campos dos Goytacazes, Brazil

[6] Ctr Univ Redentor, UniREDENTOR, Itaperuna, Brazil

[7] Ctr Diagnost & Pesquisa Osteoporose Espirito Sant, Vitoria, Brazil

[8] Univ Fed Espirito Santo, Lab Endocrinol & Cellular Toxicol, Programa Pos Grad Ciencias Fisiol, Dept Morphol,Dept Physiol Sci,Hlth Sci Ctr, Vitoria, Brazil

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2018年 / 51卷 / 01期

关键词：

Ovariectomized; Vitamin K; Bone Mineral Density; Bone Microarchitecture; Mineral Metabolism; POSTMENOPAUSAL WOMEN; ESTROGEN-RECEPTOR; MINERAL DENSITY; SEX STEROIDS; MENATETRENONE; FRACTURES; OSTEOPOROSIS; METAANALYSIS; MECHANISMS; THERAPY;

D O I：

10.1159/000495234

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Osteoporosis is a bone metabolic disease that affects mostly post-menopausal women. There has been shown that vitamin K (VK) supplementation during menopause may decrease bone loss as well as risk of bone breaking. Aiming to clarify the beneficial role of VK in bone metabolism during menopause, we investigated mineral metabolism and bone ultrastructure of ovariectomized (OVX) mice. Methods: To determine the effects chronic use of VK in bone structure and mineral metabolism in OVX mice, we used several methods, such as DXA, mu CTScan, and SEM as well as biomolecular techniques, such as ELISA and qRT-PCR. In addition, complete analysis of serum hormonal and other molecules associated to bone and lipid metabolism were evaluated overview the effects of VK in menopause murine model. Results: VK treatment significantly affects Pi metabolism independently of OVX, changing Pi plasma, urinary output, balance, and Pi bone mass. Interestingly, VK also increased VLDL in mice independently of castration. In addition, VK increased compact bone mass in OVX mice when we evaluated it by DXA, histomorphometry, mu CTScanning. VK increased bone formation markers, osteocalcin, HYP- osteocalcin, and AP whereas it decreased bone resorption markers, such as urinary DPD/creatinine ratio and plasmatic TRAP. Surprisingly, SEM images revealed that VK treatment led to amelioration of microfractures observed in OVX untreated controls. In addition, SHAM operated VK treated mice exhibited higher number of migrating osteoblasts and in situ secretion of AP. OVX led to decreased to in situ secretion of AP that was restored by VK treatment. Moreover, VK treatment increased mRNA expression of bone Calbindin 28KDa independently of OVX. Conclusion: VK treatment in OVX mice exhibited beneficial effects on bone ultrastructure, mostly by altering osteoblastic function and secretion of organic bone matrix. Therefore, VK could be useful to treat osteopenic/osteoporotic patients.

引用

页码：356 / 374

页数：19

共 45 条

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