Image normalization for quantitative immunohistochemistry in digital pathology

被引：2

作者：

Van Eycke, Yves-Remi ^{[1
,3
]}

Allard, Justine ^{[2
,3
]}

Derock, Melanie ^{[3
]}

Salmon, Isabelle ^{[2
,3
]}

Debeir, Olivier ^{[1
]}

Decaestecker, Christine ^{[1
,3
]}

机构：

[1] Univ Libre Bruxelles, Lab Image Signal Proc & Acoust, Brussels, Belgium

[2] Univ Libre Bruxelles, Erasme Hosp, Dept Pathol, Brussels, Belgium

[3] ULB, Ctr Microscopy & Mol Imaging, DIAPath, Gosselies, Belgium

来源：

2016 IEEE 13TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2016年

关键词：

Biomarker; histology; image normalization; immunohistochemistry; quantification;

D O I：

10.1109/ISBI.2016.7493386

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper, we propose to adapt to immunohistochemistry (IHC) some methods proposed to normalize images from histological slices stained with hematoxylin-eosin (H&E). Our final aim is to provide a coherent quantitative characterization of IHC biomarkers across different IHC batches with possible staining variations. In contrast to H&E, IHC staining strongly varies with the tissue analyzed and the protein targeted, making image normalization challenging. To solve this problem, we added in each IHC batch a slice from a reference tissue microarray (TMA) and then digitalized it to establish an inter-batch normalization transform. A comparison of two methods adapted to the specificity of IHC-stained slides evidences some normalization requirements to make valid IHC biomarker quantification across different staining batches.

引用

页码：795 / 798

页数：4

共 7 条

[1] Requirements for the valid quantification of immunostains on tissue microarray materials using image analysis
Decaestecker, Christine
Lopez, Xavier Moles
D'Haene, Nicky
Roland, Isabelle
Guendouz, Saad
Duponchelle, Christophe
Berton, Alix
Debeir, Olivier
Salmon, Isabelle
[J]. PROTEOMICS, 2009, 9 (19) : 4478 - 4494
[2] Kothari S, 2011, I S BIOMED IMAGING, P657, DOI 10.1109/ISBI.2011.5872492
[3] A Complete Color Normalization Approach to Histopathology Images Using Color Cues Computed From Saturation-Weighted Statistics
Li, Xingyu
Plataniotis, Konstantinos N.
[J]. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2015, 62 (07) : 1862 - 1873
[4] Lopez X.Moles., 2014, Microscopy: Advances in Scientific Research and Education, V2, P776
[5] A METHOD FOR NORMALIZING HISTOLOGY SLIDES FOR QUANTITATIVE ANALYSIS
Macenko, Marc
Niethammer, Marc
Marron, J. S.
Borland, David
Woosley, John T.
Guan, Xiaojun
Schmitt, Charles
Thomas, Nancy E.
[J]. 2009 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1 AND 2, 2009, : 1107 - +
[6] Niethammer M, 2010, LECT NOTES COMPUT SC, V6357, P58, DOI 10.1007/978-3-642-15948-0_8
[7] Van Eycke YR, 2015, IEEE ENG MED BIO, P7732, DOI 10.1109/EMBC.2015.7320184

← 1 →