Combination of selective enrichment and MALDI-TOF MS for rapid detection of Streptococcus agalactiae colonisation of pregnant women

被引:3
作者
Abrok, Marianna [1 ]
Arcson, Agnes [1 ]
Lazar, Andrea [1 ]
Urban, Edit [1 ]
Deak, Judit [1 ]
机构
[1] Univ Szeged, Inst Clin Microbiol, H-6725 Szeged, Hungary
关键词
Group B Streptococcus; Sample preparation; Todd-Hewitt broth; MALDI-TOF MS; Screening; Pregnancy; GROUP-B STREPTOCOCCUS; DESORPTION IONIZATION-TIME; IDENTIFICATION; DISEASE;
D O I
10.1016/j.mimet.2015.04.010
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Sample preparation was optimized for MALDI-TOF MS directly from selective enrichment broth to detect Streptococcus agalactiae. The method was tested on 100 vaginal samples of pregnant women; positive and negative predictive values were 100 and 91%, respectively. If it indicates positivity, colonisation can be reported 18-24 h after sample collection. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:23 / 25
页数:3
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