Bioelectrochemical control of neural cell development on conducting polymers

被引：83

作者：

Collazos-Castro, Jorge E. ^{[1
]}

Polo, Jose L. ^{[2
]}

Hernandez-Labrado, Gabriel R. ^{[2
]}

Padial-Canete, Vanesa ^{[1
]}

Garcia-Rama, Concepcion ^{[1
]}

机构：

[1] Hosp Nacl Paraplej SESCAM, Neural Repair Lab, Toledo 45071, Spain

[2] Univ Castilla La Mancha, EU Ingn Tecn Ind, Toledo 45071, Spain

来源：

BIOMATERIALS | 2010年 / 31卷 / 35期

关键词：

Conducting polymers; PEDOT:PSS; Self-assembled multilayers; Cell culture; Electrochemistry; Neural cells; FIBROBLAST-GROWTH-FACTOR; POLYPYRROLE-HEPARIN; NEURITE OUTGROWTH; POLY(3,4-ETHYLENEDIOXYTHIOPHENE); SPERMINE; PROTEIN; FILMS; MICROELECTRODES; STIMULATION; ATTACHMENT;

D O I：

10.1016/j.biomaterials.2010.08.057

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Electrically conducting polymers hold promise for developing advanced neuroprostheses, bionic systems and neural repair devices. Among them, poly(3, 4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) exhibits superior physicochemical properties but biocompatibility issues have limited its use. We describe combinations of electrochemical and molecule self-assembling methods to consistently control neural cell development on PEDOT:PSS while maintaining very low interfacial impedance. Electro-adsorbed polylysine enabled long-term neuronal survival and growth on the nanostructured polymer. Neurite extension was strongly inhibited by an additional layer of PSS or heparin, which in turn could be either removed electrically or further coated with spermine to activate cell growth. Binding basic fibroblast growth factor (bFGF) to the heparin layer inhibited neurons but promoted proliferation and migration of precursor cells. This methodology may orchestrate neural cell behavior on electroactive polymers, thus improving cell/electrode communication in prosthetic devices and providing a platform for tissue repair strategies. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：9244 / 9255

页数：12

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