Studies of the Interactions between Three Protoberberine Alkaloids and Bcl-2 by Fluorescence Spectroscopy

被引：0
|
作者：
Zhang Yanxia [1 ,2 ]
Zhang Xiufeng [1 ]
Tang Yalin [1 ]
Xiang Junfeng [1 ]
Tian Mingyue [1 ,3 ]
机构：
[1] Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[3] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
来源：
ACTA CHIMICA SINICA | 2011年 / 69卷 / 02期
关键词：
berberine hydrochloride; jatrorrhizine; palmatine hydrochloride; Bcl-2; fluorescence spectroscopy; BOVINE SERUM-ALBUMIN; BERBERINE CHLORIDE; BINDING; HYDROCHLORIDE;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The interactions between Bcl-2 and three protoberberine alkaloids: berberine hydrochloride, jatrorrhizine and palmatine hydrochloride were studied by fluorescence spectroscopy. The binding constants and the number of binding sites were measured, respectively, and the quenching mechanism was proposed. The results indicated that the protoberberine alkaloids could decrease the intrinsic fluorescence of Bcl-2 mainly through static quenching. By comparing the structures of the three alkaloids, it was found that the binding affinity to Bcl-2 protein increased with molecular flexibility of the alkaloids.
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收藏
页码：247 / 251
页数：5
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