Regorafenib Induces Apoptosis and Inhibits Metastatic Potential of Human Bladder Carcinoma Cells

被引：32
|
作者：
Hsu, Fei-Ting [1 ,2 ,3 ,4 ]
Sun, Cho-Chin [5 ]
Wu, Chia-Hsing [6 ]
Lee, Yen-Ju [7 ]
Chiang, Chih-Hung [5 ,6 ,8 ]
Wang, Wei-Shu [9 ,10 ,11 ]
机构：
[1] Taipei Med Univ, Sch Med, Dept Radiol, Taipei, Taiwan
[2] Taipei Med Univ Hosp, Dept Med Imaging, Taipei, Taiwan
[3] Taipei Med Univ Hosp, Translat Lab, Dept Med Res, Taipei, Taiwan
[4] Taipei Med Univ Hosp, Res Ctr Translat Med, Taipei, Taiwan
[5] Taipei Vet Gen Hosp, Yuan Shan Su Ao Branch, Dept Urol Med Res & Educ, Yilan, Taiwan
[6] Cardinal Tien Jr Coll Healthcare & Management, Dept Nursing, New Taipei, Taiwan
[7] Kaohsiung Armed Forces Gen Hosp, Dept Surg, Zuoying Branch, Kaohsiung, Taiwan
[8] Natl Taiwan Univ Hosp, Dept Urol, Taipei, Taiwan
[9] Natl Yang Ming Univ Hosp, Dept Med, Yilan, Taiwan
[10] Natl Yang Ming Univ Hosp, Canc Med Care Ctr, Yilan, Taiwan
[11] Natl Yang Ming Univ Hosp, Sch Med, Taipei, Taiwan
来源：
ANTICANCER RESEARCH | 2017年 / 37卷 / 09期
关键词：
Regorafenib; human bladder carcinoma; apoptosis; metastatic potential; KAPPA-B ACTIVATION; HEPATOCELLULAR-CARCINOMA; UROTHELIAL CARCINOMAS; KINASE INHIBITOR; CANCER CELLS; EXPRESSION; SORAFENIB; SUPPRESSION; RECURRENCE; CYSTECTOMY;
D O I：
10.21873/anticanres.11901
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the present study was to verify the effects of regorafenib on apoptosis and metastatic potential in TSGH 8301 human bladder carcinoma cells in vitro. Cells were treated with different concentration of regorafenib for different periods of time. Effects of regorafenib on cell viability, apoptosis pathways, metastatic potential, and expression of metastatic and anti-apoptotic proteins were evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, flow cytometry, cell migration and invasion assay, and western blotting. We found regorafenib significantly reduced cell viability, cell migration and invasion, and expression of metastatic and anti-apoptotic proteins. In addition, regorafenib significantly induced accumulation of sub-G1 phase cells, loss of mitochondrial membrane potential, and expression of active caspase-3 and caspase-8. These results show that regorafenib not only induces apoptosis, but also inhibits metastatic potential in bladder cancer TSGH 8301 cells in vitro.
引用
收藏
页码：4919 / 4926
页数：8
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