Differential Expression of Flavonoid Biosynthesis Genes and Accumulation of Phenolic Compounds in Common Buckwheat (Fagopyrum esculentum)

被引：98

作者：

Li, Xiaohua ^{[1
]}

Park, Nam Il ^{[1
]}

Xu, Hui ^{[1
]}

Woo, Sun-Hee ^{[2
]}

Park, Cheol Ho ^{[3
]}

Park, Sang Un ^{[1
]}

机构：

[1] Chungnam Natl Univ, Coll Agr & Life Sci, Dept Crop Sci, 220 Gung Dong, Taejon 305754, South Korea

[2] Chungbuk Natl Univ, Dept Crop Sci, Coll Agr Life & Environm Sci, Cheongju 361763, South Korea

[3] Kangwon Natl Univ, Dept Biohlth Technol, Coll Biomed Sci, Chunchon 200701, South Korea

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 23期

关键词：

Common buckwheat; Fagopyrum esculentum; flavonoids; rutin; biosynthetic pathways; gene expression; BIOCHEMISTRY; GENETICS; RUTIN; FLAVANONE; SEEDLINGS; PLANTS; ACID;

D O I：

10.1021/jf103310g

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Common buckwheat (Fagopyrum esculentum) is a short-season grain crop that is a source of rutin and other phenolic compounds. In this study, we isolated the cDNAs of 11 F. esculentum enzymes in the flavonoid biosynthesis pathway, namely, phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL) 1 and 2, chalcone synthase (CHS), chalcone isomerase (CHI), flavone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) 1 and 2, and anthocyanidin synthase (ANS). Quantitative real-time polymerase chain reaction analysis showed that these genes were most highly expressed in the stems and roots. However, high performance liquid chromatography analysis indicated that their flavonoid products, such as rutin and catechin, accumulated in the flowers and leaves. These results suggested that flavonoids may be transported within F. esculentum. In addition, light and dark growth conditions affected the expression levels of the biosynthesis genes and accumulation of phenolic compounds in F. esculentum sprouts.

引用

页码：12176 / 12181

页数：6

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