Isolation of a novel N-acetylglucosamine-specific lectin from fresh sclerotia of the edible mushroom Pleurotus tuber-regium

被引：28
作者：
Wang, HX
Ng, TB [1 ]
机构：
[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing, Peoples R China
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2003年 / 29卷 / 02期
关键词：
lectin; mushroom sclerotia; Pleurotus tuber-regium;
D O I：
10.1016/S1046-5928(03)00059-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An N-acetylglucosamine-binding lectin with a molecular mass of 32 kDa was isolated from fresh sclerotia of the edible mushroom Pleurotus tuber-regium. Its N-terminal sequence exhibited some similarity to that of Agaricus bisporus lectin. The isolation procedure was simple, involving (NH4)(2)SO4 precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on N-acetyl-D-glucosamine-agarose, and gel filtration by fast protein liquid chromatography on Superdex 75. The lectin exhibited hemagglutinating activity toward trypsinized rabbit erythrocytes but not toward untrypsinized rabbit erythrocytes. (C) 2003 Elsevier Science (USA). All rights reserved.
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收藏
页码：156 / 160
页数：5
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