Dendrimer type bio-reducible polymer for efficient gene delivery

被引：70

作者：

Nam, Hye Yeong ^{[2
]}

Nam, Kihoon ^{[2
]}

Lee, Minhyung ^{[3
]}

Kim, Sung Wan ^{[2
]}

Bull, David A. ^{[1
]}

机构：

[1] Univ Utah, Hlth Sci Ctr, Div Cardiothorac Surg, Dept Surg,Sch Med, Salt Lake City, UT 84132 USA

[2] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, CCCD, Salt Lake City, UT 84112 USA

[3] Hanyang Univ, Coll Engn, Dept Bioengn, Seoul 133791, South Korea

来源：

JOURNAL OF CONTROLLED RELEASE | 2012年 / 160卷 / 03期

关键词：

Bioreducible polymer; Dendrimer; Gene delivery; Transfection; Cytotoxicity; ENHANCED TRANSFECTION EFFICIENCY; GLUTATHIONE DEPLETION; DEGRADATION; ARGININE;

D O I：

10.1016/j.jconrel.2012.04.025

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：592 / 600

页数：9

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