Formation of the Secondary Abscission Zone Induced by the Interaction of Methyl Jasmonate and Auxin in Bryophyllum calycinum: Relevance to Auxin Status and Histology

被引:12
|
作者
Marasek-Ciolakowska, Agnieszka [1 ]
Saniewski, Marian [1 ]
Dziurka, Michal [2 ]
Kowalska, Urszula [1 ]
Goraj-Koniarska, Justyna [1 ]
Ueda, Junichi [3 ]
Miyamoto, Kensuke [4 ]
机构
[1] Res Inst Hort, Konstytucji 3 Maja 1-3, PL-96100 Skierniewice, Poland
[2] Polish Acad Sci, Franciszek Gorski Inst Plant Physiol, Niezapominajek 21, PL-30239 Krakow, Poland
[3] Osaka Prefecture Univ, Grad Sch Sci, Dept Biol Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
[4] Osaka Prefecture Univ, Fac Liberal Arts & Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
关键词
auxin; abscission; Bryophyllum calycinum; histology; hormonal crosstalk; methyl jasmonate; stem; STEM ABSCISSION; INDOLE-3-ACETIC-ACID CATABOLITE; ARABIDOPSIS-THALIANA; IMPATIENS-SULTANI; LEAF ABSCISSION; ACID; EXPLANTS; TRANSDIFFERENTIATION; HOMEOSTASIS; PHASEOLUS;
D O I
10.3390/ijms21082784
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interaction of methyl jasmonate (JA-Me) and indole-3-acetic acid (IAA) to induce the formation of the secondary abscission zone in the middle of internode segments of Bryophyllum calycinum was investigated in relation to auxin status and histology. When IAA at 0.1% (w/w, in lanolin) was applied to the segments, the formation of the secondary abscission zone at a few mm above the treatment in the apical direction was observed. On the contrary, IAA at 0.5% (w/w, in lanolin) did not induce the formation of the secondary abscission zone. JA-Me at 0.5% (w/w, in lanolin) applied to the middle of internode segments kept in the normal (natural) or inverted positions also induced the formation of the secondary abscission zone below and above parts of the treatment. IAA at 0.5% applied to the cut surface of the upper part of the segments completely prevented the formation of the secondary abscission zone induced by JA-Me. Simultaneous application of IAA 0.5% with JA-Me 0.5% in the middle part of the internode segments induced the formation of the secondary abscission zone at 10 mm to 12 mm above the treatment. Histological analyses indicated that the formation of the secondary abscission zone was characterized by the presence of newly synthesized cell plates that resulted from periclinal cell division within one layer of mother cells in stems. The effects of IAA (0.1%) and JA-Me (0.5%) on the formation of the secondary abscission zone were histologically similar. Comprehensive analyses of plant hormones revealed that the balance of the endogenous levels of IAA in both sides adjacent to the abscission zone was significantly disturbed when the secondary abscission formation was induced by the application of IAA. These results strongly suggest that an auxin gradient is important in the formation of the secondary abscission zone in the internode segments of B. calycinum, and IAA gradient results from polar IAA transport from the application site. IAA is important in the regulation of formation of the secondary abscission zone induced by JA-Me. Further possible mechanisms of the formation of the secondary abscission zone in the internode segments of B. calycinum are also discussed in the interaction of JA-Me and IAA.
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页数:18
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