Development and Validation of UV Spectrophotometric Method for Determination of Chrysin and Its Solubility Studies

The present work is based on the method development and validation of the UV-spectrophotometric method for the quantitative estimation of chrysin. We studied how the nature of the solvent affects chrysin solubility. The stock and diluted solutions were scanned using a UV visible spectrophotometer to obtain the lambda(max). The absorbance of the samples was recorded to obtain a calibration curve, which was followed by regression using MS Excel and Sigma stat software. The method developed by this process was further validated using parameters such as linearity, precision, limit of detection, limit of quantification, accuracy, and ruggedness. The solubility of chrysin was checked at 35 degrees C in different oils, solvents, and co-solvents. The lambda(max) of chrysin in methanol was found to be 367 nm. The calibration curve of the drug follows linearity (2-10 mu g/mL) with a correlation coefficient of 0.991. At three different levels, i.e., 80, 100, and 120%, the method's accuracy was checked utilizing the percent recovery (97-99.5%). The precision studies were carried out in terms of intraday and interday variations. The ruggedness of the proposed method was studied by taking two. The solubility of chrysin was found to be the maximum in methanol (216.80 +/- 0.0097 mu g/mL) among the oils, solvents, and co-solvents used. Thus, based on the experiments done, the developed method was observed to be accurate, precise, and reproducible. The viscosity of the solvent and the possibility of hydrogen bonding are two crucial factors that affected the solubility of chrysin in the solvents.