Enforced Bipedal Downhill Running Induces Achilles Tendinosis in Rats

被引:33
作者
Ng, Gabriel Yin-Fat [1 ]
Chung, Polly Yee-Man [1 ]
Wang, Jenny Shijie [2 ]
Cheung, Roy Tsz-Hei [1 ]
机构
[1] Hong Kong Polytech Univ, Dept Rehabil Sci, Kowloon, Hong Kong, Peoples R China
[2] Xi An Jiao Tong Univ, Dept Pathophysiol, Sch Med, Xian 710049, Peoples R China
关键词
Achilles tendon; tendinopathy; downhill running; strength; degeneration; INTRINSIC RISK-FACTORS; OVERUSE ACTIVITY; ANIMAL-MODEL; TENDON; TENDINOPATHY; LIGAMENT; PERFORMANCE; STRENGTH; INJURY; LASER;
D O I
10.3109/03008207.2011.562334
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Enforced downhill running has been reported to induce tendinosis in the rat supraspinatus tendon but similar exercise failed to induce Achilles tendinosis in this animal. Due to the presence of acromial arch in the shoulder, accessing the supraspinatus tendon with physical modalities is difficult; thus this model may not be suitable for studying the treatment for tendinosis. To develop a rat model for Achilles tendinosis, we tested 14 mature Sprague-Dawley rats by dividing them into 2 groups of 7 each. The experimental group was subjected to a daily enforced downhill bipedal running program by suspending their upper bodies so that they ran with their hind limbs on a treadmill for 1 hr/day for 8 weeks. The downward inclination was 20 and the speed was 17 m/min. The animals in the control group did not undergo any exercise. After 8 weeks, the Achilles tendons were harvested and subjected to histological and biomechanical analysis. Histological examination revealed tenocyte proliferation, change in tenocytes appearance, and collagen bundle disintegration in the running group. The biomechanical testing revealed significant decrease in stiffness (p = 0.002) and ultimate tensile strength (p = 0.016) in the running group than in the control group. Both the histological and biomechanical findings are suggestive of changes in the tendon of the running group that resembled the pathological changes of tendinosis in human. This new model of Achilles tendinosis in rat will be useful for studying the etiology and subsequent management strategies of this condition.
引用
收藏
页码:466 / 471
页数:6
相关论文
共 27 条
[1]  
Archambault JM, 2005, TENDON INJURIES: BASIC SCIENCE AND CLINICAL MEDICINE, P279, DOI 10.1007/1-84628-050-8_26
[2]   Effects of low-voltage microamperage stimulation on tendon healing in rats [J].
Chan, Helen K. F. ;
Fung, Dicky T. C. ;
Ng, Gabriel Y. F. .
JOURNAL OF ORTHOPAEDIC & SPORTS PHYSICAL THERAPY, 2007, 37 (07) :399-403
[3]   Abnormal tenocyte morphology is more prevalent than collagen disruption in asymptomatic athletes' patellar tendons [J].
Cook, JL ;
Feller, JA ;
Bonar, SF ;
Khan, KM .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2004, 22 (02) :334-338
[4]   Anthropometry, physical performance, and ultrasound patellar tendon abnormality in elite junior basketball players: a cross-sectional study [J].
Cook, JL ;
Kiss, ZS ;
Khan, KM ;
Purdam, CR ;
Webster, KE .
BRITISH JOURNAL OF SPORTS MEDICINE, 2004, 38 (02) :206-209
[5]   Exposure-Dependent Increases in IL-1β, Substance P, CTGF, and Tendinosis in Flexor Digitorum Tendons with Upper Extremity Repetitive Strain Injury [J].
Fedorczyk, Jane M. ;
Barr, Ann E. ;
Rani, Shobha ;
Gao, Helen G. ;
Amin, Mamta ;
Amin, Shreya ;
Litvin, Judith ;
Barbe, Mary F. .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2010, 28 (03) :298-307
[6]   SKELETAL STRUCTURAL ADAPTATIONS TO MECHANICAL USAGE (SATMU) .4. MECHANICAL INFLUENCES ON INTACT FIBROUS TISSUES [J].
FROST, HM .
ANATOMICAL RECORD, 1990, 226 (04) :433-439
[7]   Therapeutic low energy laser improves the mechanical strength of repairing medial collateral ligament [J].
Fung, DTC ;
Ng, GYF ;
Leung, MCP ;
Tay, DKC .
LASERS IN SURGERY AND MEDICINE, 2002, 31 (02) :91-96
[8]   Histological analysis of Achilles tendons in an overuse rat model [J].
Glazebrook, Mark A. ;
Wright, James R., Jr. ;
Langman, Maxine ;
Stanish, William D. ;
Lee, J. Michael .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2008, 26 (06) :840-846
[9]   The effect of overuse activity on Achilles tendon in an animal model: A biomechanical study [J].
Huang, TF ;
Perry, SM ;
Soslowsky, LJ .
ANNALS OF BIOMEDICAL ENGINEERING, 2004, 32 (03) :336-341
[10]   Health of master track and field athletes - A 16-year follow-up study [J].
Kettunen, JA ;
Kujala, UM ;
Kaprio, J ;
Sarna, S .
CLINICAL JOURNAL OF SPORT MEDICINE, 2006, 16 (02) :142-148