Graphene Oxide-Peptide Conjugate as an Intracellular Protease Sensor for Caspase-3 Activation Imaging in Live Cells

被引：299

作者：

Wang, Haibo ^{[1
]}

Zhang, Qing ^{[1
]}

Chu, Xia ^{[1
]}

Chen, Tingting ^{[1
]}

Ge, Jia ^{[1
]}

Yu, Ruqin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 31期

关键词：

biosensors; caspase; fluorescent probes; graphene; peptides; GOLD NANOPARTICLES; ENERGY-TRANSFER; APOPTOSIS; MOLECULE; DEATH; DELIVERY; PLATFORM; NUCLEUS; DNA;

D O I：

10.1002/anie.201101351

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

All systems GO! An intracellular protease sensor is based on the covalent conjugate of graphene oxide and peptide substrates with fluorophore labels. The conjugate can be delivered into live cells and provides specific, high-contrast imaging of caspase-3 activation (see picture; orange=cell penetration peptide, blue/black=caspase-3 peptide probe). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7065 / 7069

页数：5

共 35 条

[1]

[Anonymous], 2009, ANGEW CHEM

[2]

Balapanuru J., 2009, ANGEW CHEM, V121, P4879, DOI [10.1002/ange.200901479, DOI 10.1002/ange.200901479]

[3]

Balapanuru J., 2010, Angew. Chem, V122, P6699, DOI DOI 10.1002/ANGE.201001004

[4] A Graphene Oxide-Organic Dye Ionic Complex with DNA-Sensing and Optical-Limiting Properties [J].

Balapanuru, Janardhan ;

Yang, Jia-Xiang ;

Xiao, Si ;

Bao, Qiaoliang ;

Jahan, Maryam ;

Polavarapu, Lakshminarayana ;

Wei, Ji ;

Xu, Qing-Hua ;

Loh, Kian Ping .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (37) :6549-6553

[5] Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus [J].

de la Fuente, JM ;

Berry, CC .

BIOCONJUGATE CHEMISTRY, 2005, 16 (05) :1176-1180

[6]

Dreyer D. R., 2010, ANGEW CHEM, V122, P9524

[7] From Conception to Realization: An Historial Account of Graphene and Some Perspectives for Its Future [J].

Dreyer, Daniel R. ;

Ruoff, Rodney S. ;

Bielawski, Christopher W. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (49) :9336-9344

[8]

Giljohann D.A., 2010, Angew. Chem, V122, P3352, DOI DOI 10.1002/ANGE.200904359

[9] Gold Nanoparticles for Biology and Medicine [J].

Giljohann, David A. ;

Seferos, Dwight S. ;

Daniel, Weston L. ;

Massich, Matthew D. ;

Patel, Pinal C. ;

Mirkin, Chad A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (19) :3280-3294

[10] Apoptotic pathways: The roads to ruin [J].

Green, DR .

CELL, 1998, 94 (06) :695-698

← 1 2 3 4 →