Cloning of a river pufferfish (Takifugu obscurus) metallothionein cDNA and study of its induction profile in cadmium-exposed fish

被引：44

作者：

Kim, Jin-Hyoung ^{[2
]}

Wang, Soon-Young ^{[2
]}

Kim, Il-Chan ^{[3
]}

Ki, Jang-Seu ^{[1
]}

Raisuddin, Sheikh ^{[1
]}

Lee, Jae-Seong ^{[1
]}

Han, Kyung-Nam ^{[2
]}

机构：

[1] Hanyang Univ, Coll Nat Sci, Natl Res Lab Marine Mol & Environm Biosci, Dept Chem, Seoul 133791, South Korea

[2] Inha Univ, Coll Nat Sci, Dept Marine Sci, Inchon 402751, South Korea

[3] Korea Ocean Res & Dev Inst, Korea Polar Res Inst, Polar BioCtr, Inchon 406840, South Korea

来源：

CHEMOSPHERE | 2008年 / 71卷 / 07期

关键词：

Takifugu obscurus; metallothionein; cadmium; detoxification; biomarker;

D O I：

10.1016/j.chemosphere.2007.11.067

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We report here the full-length cDNA sequence of metallothionein (MT) gene from ail anadromous river pufferfish, Takifugu obscurus (order: Tetradotiformes; family: Tetradontidae). Phylogenetic relationship analysis revealed that the identified MT has high sequence similarity with many Perciformes fish species. The tissue distribution and concentration- and time-dependent expression of MT mRNA were studied in fish exposed to cadmium. Liver showed the highest level of MT gene expression followed by other tissues (brain, gill and kidney) in response to cadmium exposure. Muscle showed a weak expression response of MT gene. Time-course study revealed highest early phase (at 6 h) expression in the brain and late persistence of induction in the intestine. MT mRNA expression showed a concentration-dependent expression in all the tissues. However, induction in brain and liver occurred at much lower concentrations as compared to other tissues. Our results demonstrate that MT in T obscurus is induced by cadmium exposure which indicates that it plays a functionally conserved function of metal detoxification. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1251 / 1259

页数：9

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