Cross-protective efficacies of highly-pathogenic avian influenza H5N1 vaccines against a recent H5N8 virus

被引：13

作者：

Park, Su-Jin ^{[1
,2
]}

Si, Young-Jae ^{[1
,2
]}

Kim, Jihye ^{[3
]}

Song, Min-Suk ^{[1
,2
]}

Kim, Se-mi ^{[1
,2
]}

Kim, Eun-Ha ^{[1
,2
]}

Kwon, Hyeok-il ^{[1
,2
]}

Kim, Young-Il ^{[1
,2
]}

Lee, Ok-Jun ^{[1
,2
]}

Shin, Ok Sarah ^{[4
]}

Kim, Chul-Joong ^{[5
]}

Shin, Eui-Cheol ^{[3
]}

Choi, Young Ki ^{[1
,2
]}

机构：

[1] Chungbuk Natl Univ, Coll Med, Chungdae Ro 1, Cheongju 28644, South Korea

[2] Chungbuk Natl Univ, Med Res Inst, Chungdae Ro 1, Cheongju 28644, South Korea

[3] Korea Adv Inst Sci & Technol, Lab Immunol & Infect Dis, Grad Sch Med Sci & Engn, Daehak Ro 291, Daejeon 34141, South Korea

[4] Korea Univ, Dept Microbiol, Coll Med, Seoul 136701, South Korea

[5] Chungnam Natl Univ, Coll Vet Med, Daehak Ro 99, Daejeon 34134, South Korea

来源：

VIROLOGY | 2016年 / 498卷

关键词：

HPAI H5N1; H5N8; Vaccine; ADCC; Cross-protection; WHOLE-VIRUS; A-VIRUS; ANTIBODY; CHALLENGE; IMMUNOGENICITY; CYTOTOXICITY; SPLIT; CELLS; MICE;

D O I：

10.1016/j.virol.2016.08.010

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To investigate cross-protective vaccine efficacy of highly-pathogenic avian influenza H5N1 viruses against a recent HPAI H5N8 virus, we immunized C57BL/6 mice and ferrets with three alum-adjuvanted inactivated whole H5N1 vaccines developed through reverse-genetics (Rg): [Vietnam/1194/04xPR8 (clade 1), Korea/W149/06xPR8 (clade 2.2), and Korea/ES223N/03xPR8 (clade 2.5)]. Although relatively low cross-reactivities (10-40 HI titer) were observed against heterologous H5N8 virus, immunized animals were 100% protected from challenge with the 20 mLD(50) of H5N8 virus, with the exception of mice vaccinated with 3.5 mu g of Rg Vietnam/1194/04xPR8. Of note, the Rg Korea/ES223N/03xPR8 vaccine provided not only effective protection, but also markedly inhibited viral replication in the lungs and nasal swabs of vaccine recipients within five days of HPAI H5N8 virus challenge. Further, we demonstrated that antibody-dependent cell-mediated cytotoxicity (ADCC) of an antibody-coated target cell by cytotoxic effector cells also plays a role in the heterologous protection of H5N1 vaccines against H5N8 challenge. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：36 / 43

页数：8

共 43 条

[1]

[Anonymous], SUMM STAT DEV AV A H

[2]

[Anonymous], 2015, COMP HDB WHO GUID PR

[3] Phylogenetic analysis of highly pathogenic avian influenza A(H5N8) virus outbreak strains provides evidence for four separate introductions and one between-poultry farm transmission in the Netherlands, November 2014 [J].