Haemoglobin expression in human endometrium

被引:51
作者
Dassen, H. [1 ,2 ]
Kamps, R. [1 ,2 ]
Punyadeera, C. [1 ,2 ]
Dijcks, F. [3 ]
de Goeij, A. [1 ,2 ]
Ederveen, A. [3 ]
Dunselman, G. [1 ,3 ,4 ]
Groothuis, P. [1 ,4 ]
机构
[1] Univ Maastricht, Univ Hosp Maastricht, Res Inst GROW, NL-6229 HX Maastricht, Netherlands
[2] Univ Maastricht, Univ Hosp Maastricht, Dept Pathol, NL-6229 HX Maastricht, Netherlands
[3] NV Organon, Dept Pharmacol, NL-5340 BH Oss, Netherlands
[4] Univ Maastricht, Univ Hosp Maastricht, Dept Obstet & Gynaecol, NL-6229 HX Maastricht, Netherlands
关键词
haemoglobin; haeme oxygenase; human endometrium; menstrual cycle; iron;
D O I
10.1093/humrep/dem430
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
BACKGROUND: The general concept that haemoglobin is only a carrier protein for oxygen and carbon dioxide is challenged since recent studies have shown haemoglobin expression in non-erythroid cells and the protection of haemoglobin against oxidative and nitrosative stress. Using microarrays, we previously showed expression of haemoglobins alpha, beta, delta and gamma and the haeme metabolizing enzyme, haeme oxygenase (HO)-1 in human endometrium. METHODS: Using real-time quantitative PCR, haemoglobin alpha, beta, delta and gamma, and HO-1 mRNA levels were assessed throughout the menstrual cycle (n = 30 women). Haemoglobin and HO-1 protein levels in the human endometrium were assessed with immunohistochemistry. For steroid responsiveness, menstrual and late proliferative-phase endometrial explants were cultured for 24 h in the presence of vehicle (0.1% ethanol), estradiol (17 beta-E-2, 1 nM), progestin (Org 2058, 1 nM) or 17 beta-E-2+Org 2058 (1 nM each). RESULTS: All haemoglobins and the HO-1 were expressed in normal human endometrium. Haemoglobin mRNA and protein expression did not vary significantly during the menstrual cycle. Explant culture with Org 2058 or 17 beta-E-2+Org 2058 increased haemoglobin gamma mRNA expression (P < 0.05). HO-1 mRNA levels, and not protein levels, were significantly higher during the menstrual (M)-phase of the cycle (P < 0.05), and were down-regulated by Org 2058 in M-phase explants and by 17 beta-E-2+Org 2058 in LP-phase explants, versus control (P < 0.05). CONCLUSIONS: The haemoglobin-HO-1 system may be required to ensure adequate regulation of the bioavailability of haeme, iron and oxygen in human endometrium.
引用
收藏
页码:635 / 641
页数:7
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