Optimization of a culture medium based on forage palm for δ-endotoxin production

被引：2

作者：

Freire da Silva, Tulio Alexandre ^{[1
]}

de Freitas, Livia Santos ^{[1
]}

Jose Bezerra da Silva, Larita Veruska ^{[2
]}

Wanderley Duarte Neto, Jose Manoel ^{[2
]}

da Silva, Gilvanda Ribeiro ^{[2
]}

de Almeida Castro Maranhao, Liane Maria ^{[2
]}

Braga de Castro Coitinho, Rodrigo Leandro ^{[2
]}

de Lacerda, Cynthia Araujo ^{[2
]}

Oliveira, Jose de Paula ^{[2
]}

Bezerra, Raquel Pedrosa ^{[1
]}

Figueiredo Porto, Ana Lucia ^{[1
]}

机构：

[1] Fed Rural Univ Pernambuco UFRPE, Dept Morphol & Anim Physiol, Ave Dom Manoel de Medeiros, BR-52171900 Recife, PE, Brazil

[2] Agron Inst Pernambuco IPA, Ave Gen San Martin 1371, BR-50761000 Recife, PE, Brazil

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2020年 / 27卷

关键词：

Spodoptera frugiperda; Bacillus thuringiensis; Inorganic nitrogen source; Bioinsecticide; Biological control; Agricultural pests; THURINGIENSIS-BASED BIOPESTICIDES; BACILLUS-THURINGIENSIS; FERMENTATION; ISRAELENSIS; EXTRACTION; PESTICIDES; KURSTAKI; SPORES; WATER; ACID;

D O I：

10.1016/j.bcab.2020.101664

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An alternative culture medium based on forage palm (Cactaceae) and inexpensive nitrogen sources for delta-endotoxin production by Bacillus thuringiensis var. berliner (Btb) was optimized using response surface methodology and the influence of the nitrogen sources on biological activity and morphological properties of the delta-endotoxin has been studied. Effects of forage palm extract concentration (15-25%), ammonium sulphate (0-1 g/L), ammonium chloride (0-1 g/L) and urea concentrations (0-1 g/L) on delta-endotoxin production were studied by a 2(4) full factorial design and optimized by a 2(2) central composite design (CCD). Statistical analysis showed that forage palm and ammonium sulphate were the main factors to enhance delta-endotoxin production. Different nitrogen sources influenced on biological activity and morphology of crystals. Culture medium constituted by forage palm extract and ammonium sulphate increased delta-endotoxins production in the form of bipyramidal crystals and was more toxic to Spodoptera frugiperda. Forage palm extract and ammonium sulphate were considered to optimization of delta-endotoxin production. Maximum delta-endotoxin production (356 mg/L) took place at 30.7% (w/v) of forage palm extract concentration and 1.016 g/L of ammonium sulphate.

引用

页数：8

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