Determination of glucose by enzyme fluorescence capillary analytical method

A method was developed for the quick determination of glucose using fluorescence capillary analysis (FCA). The experimental conditions were as follows: reaction time, 15 min; temperature: 30 degrees C; pH of phosphate buffer solution: 6.0; the concentrations of herseradish peroxidase (HRP) and glucosse oxidase (GOD) were 200 U/L and 150 U/L, respectively. The linear range of FCA was 0.1 - 4 mg/L, the relative standard deviation (RSD) was < 1.2%, and the detection limit of glucose was 0.07 mg/L. The method has been applied for the determination of glucose in blood, the recovery was 98.9% - 103.8%. Compared with the other methods, the FCA had a more simple operation. A tiny sample volume was required (only 18 mu L), so it has the advantage for saving the enzyme reagent.