Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin

被引：30

作者：

Ding, Yao

Gelfenbeyn, Kirill

Freire-de-Lima, Leonardo

Handa, Kazuko

Hakomori, Sen-itiroh ^{[1
]}

机构：

[1] Pacific NW Res Inst, Div Biomembrane Res, Seattle, WA 98122 USA

来源：

FEBS LETTERS | 2012年 / 586卷 / 13期

关键词：

Oncofetal FN; O-GalNAc; EMT; Non-small cell lung cancer; TGF beta 1; MONOCLONAL-ANTIBODY FDC-6; CELL MOTILITY; MALIGNANCY; REVERSION; INSIGHTS; HEALTH; FORM;

D O I：

10.1016/j.febslet.2012.05.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)beta 1 in EMT induction. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1813 / 1820

页数：8

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