A protein of the leucine-rich repeats (LRRs) superfamily is implicated in antimony resistance in Leishmania infantum amastigotes

被引：14
作者：
Genest, Paul-Andre
Haimeur, Anass
Legare, Danielle
Sereno, Denis
Roy, Gaetan
Messier, Nadine
Papadopoulou, Barbara
Ouellette, Marc
机构：
[1] CHU Laval, Ctr Rech & Infect, Ctr Rech, Quebec City, PQ G1V 4G2, Canada
[2] Univ Laval, Fac Med, Dept Microbiol, Quebec City, PQ G1K 7P4, Canada
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2008年 / 158卷 / 01期
基金：
加拿大健康研究院;
关键词：
Leishmania amastigotes; functional cloning; drug resistance; leucine-rich repeats;
D O I：
10.1016/j.molbiopara.2007.11.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pentavalent antimonial containing drugs (SbV) are the mainstay for the control of the protozoan parasite Leishmania but resistance to this class of drug is now prevalent in several endemic areas. We describe here the use of functional cloning where an expression cosmid bank derived from Leishmania infantum was transfected in L. infantum axenic amastigotes and selected for potassium antimonyl tartrate (SbIII) resistance. This strategy allowed the isolation of a cosmid encoding for a novel resistance protein, LinJ34.0570, which belongs to the superfamily of leucine-rich repeat (LRR) proteins. Parasites overexpressing this LRR protein, which is part of the LRR-CC subfamily, were resistant to SbIII as axenic amastigotes and to SbV as intracellular parasites. This work pinpoints a novel protein that can contribute to antimonial resistance in Leishmania. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：95 / 99
页数：5
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