Fully automated spatially resolved reflectance spectrometer for the determination of the absorption and scattering in turbid media

被引:50
作者
Foschum, F. [1 ]
Jaeger, M. [1 ]
Kienle, A. [1 ]
机构
[1] Univ Ulm, Inst Lasertechnol Med & Messtech, D-89081 Ulm, Germany
关键词
TISSUE OPTICAL-PROPERTIES; STEADY-STATE; LIGHT; TRANSMITTANCE; CALIBRATION;
D O I
10.1063/1.3648120
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
We describe a fully automated setup which is based on measurements of the spatially resolved reflectance for the determination of the reduced scattering and absorption coefficients in semi-infinite turbid media. The sample is illuminated with a xenon light source in combination with a monochromator enabling the scan of the wavelength from 450 nm to 950 nm. Reflected light from the sample is detected with a CCD camera providing a high spatial resolution. The essential steps for signal processing including, e.g., the consideration of the optical transfer function and the correct treatment of the background subtraction, are presented. The solutions of the diffusion theory and of the radiative transfer theory are investigated regarding the exact detection and illumination geometry. Systematic errors caused by using the different theories for fitting the optical parameters are characterized. The system was validated using liquid phantoms which contain Intralipid 20% and ink, and the measurement range of the system is specified. Further, we carefully characterized the optical properties of Intralipid 20% in the wavelength range between 450 nm and 950 nm. (C) 2011 American Institute of Physics. [doi:10.1063/1.3648120]
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页数:10
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共 32 条
[1]   Evaluation of a novel noncontact spectrally and spatially resolved reflectance setup with continuously variable source-detector separation using silicone phantoms [J].
Andree, Stefan ;
Reble, Carina ;
Helfmann, Juergen ;
Gersonde, Ingo ;
Illing, Gerd .
JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (06)
[2]   METHOD FOR MEASURING POSITION-DEPENDENT VOLUME REFLECTION [J].
BOLT, RA ;
TENBOSCH, JJ .
APPLIED OPTICS, 1993, 32 (24) :4641-4645
[3]   ON THE DETERMINATION OF OPTICAL-PARAMETERS FOR TURBID MATERIALS [J].
BOLT, RA ;
TENBOSCH, JJ .
WAVES IN RANDOM MEDIA, 1994, 4 (03) :233-242
[4]   The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopy [J].
Doornbos, RMP ;
Lang, R ;
Aalders, MC ;
Cross, FW ;
Sterenborg, HJCM .
PHYSICS IN MEDICINE AND BIOLOGY, 1999, 44 (04) :967-981
[5]   Effects of point-spread function on calibration and radiometric accuracy of CCD camera [J].
Du, H ;
Voss, KJ .
APPLIED OPTICS, 2004, 43 (03) :665-670
[6]   USE OF HOUGH TRANSFORMATION TO DETECT LINES AND CURVES IN PICTURES [J].
DUDA, RO ;
HART, PE .
COMMUNICATIONS OF THE ACM, 1972, 15 (01) :11-&
[7]   Frequency-domain photon migration measurements of normal and malignant tissue optical properties in a human subject [J].
Fishkin, JB ;
Coquoz, O ;
Anderson, ER ;
Brenner, M ;
Tromberg, BJ .
APPLIED OPTICS, 1997, 36 (01) :10-20
[8]   OPTICAL-PROPERTIES OF INTRALIPID - A PHANTOM MEDIUM FOR LIGHT-PROPAGATION STUDIES [J].
FLOCK, ST ;
JACQUES, SL ;
WILSON, BC ;
STAR, WM ;
VANGEMERT, MJC .
LASERS IN SURGERY AND MEDICINE, 1992, 12 (05) :510-519
[9]   SCATTERING AND ABSORPTION OF TURBID MATERIALS DETERMINED FROM REFLECTION MEASUREMENTS .2. MEASURING METHOD AND CALIBRATION [J].
GROENHUIS, RAJ ;
TENBOSCH, JJ ;
FERWERDA, HA .
APPLIED OPTICS, 1983, 22 (16) :2463-2467
[10]   SCATTERING AND ABSORPTION OF TURBID MATERIALS DETERMINED FROM REFLECTION MEASUREMENTS .1. THEORY [J].
GROENHUIS, RAJ ;
FERWERDA, HA ;
TENBOSCH, JJ .
APPLIED OPTICS, 1983, 22 (16) :2456-2462