Cytotoxic activity of withanolides isolated from Tunisian Datura metel L.

被引：39

作者：

Bellila, Amor ^{[2
]}

Tremblay, Cindy ^{[1
]}

Pichette, Andre ^{[1
]}

Marzouk, Brahim ^{[2
]}

Mshvildadze, Vakhtang ^{[1
]}

Lavoie, Serge ^{[1
]}

Legault, Jean ^{[1
]}

机构：

[1] Univ Quebec Chicoutimi, Dept Sci Fondamentales, Chaire Rech Agents Anticanc Origine Nat, Chicoutimi, PQ G7H 2B1, Canada

[2] Biotechnol Ctr Borj Cedria Technopk, Aromat & Med Plants Unit, Hammam Lif 901, Tunisia

来源：

PHYTOCHEMISTRY | 2011年 / 72卷 / 16期

关键词：

Datura metel L; Solanaceae; Cytotoxic activity; Steroids; Withametelin Q; 12 alpha-Hydroxydaturametelin B; ANTIMICROBIAL ACTIVITY; SOLANACEOUS PLANTS; CONSTITUENTS; SCOPOLAMINE; APOPTOSIS;

D O I：

10.1016/j.phytochem.2011.07.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Withanolide-type steroids, withametelin Q (1) and 12 alpha-hydroxydaturametelin B (2) along with three known withanolides, were isolated from leaves of Datura metel L. (Solanaceae). The respective structures, characterized mainly by NMR spectroscopy, were identified as (20R,22R,24R)-21,24-epoxy-1 alpha,3 beta-dihydroxywitha-5,25(27)-dienolide-3-O-beta-D-glucopyranoside (1) and (20R,22R,24R)-12 alpha,21,27-trihydroxy-1-oxowitha-2,5,24-trienolide-27-O-beta-D-glucopyranoside (2). The cytotoxicity of isolated compounds was evaluated against human lung carcinoma cells (A549) and human colorectal adenocarcinoma cells (DLD-1), respectively. Compound 2 exhibited cytotoxicity against A549 and DLD-1 cell lines, with IC50 values of 7 and 2.0 mu M, respectively. However, for compounds 6 and 7, cytotoxicities were higher against DLD-1 cells with IC50 values of 0.6 and 0.7 mu M. Both compounds blocked the cell cycle in the S-phase and induced apoptosis. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：2031 / 2036

页数：6

共 29 条

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