Association between single-nucleotide polymorphisms in ARID4A gene and sperm quality of Chinese water buffalo

被引：0

作者：

Lu, Guanhong ^{[1
,2
]}

He, Chuanyu ^{[3
]}

Yang, Xi

Cheng, Yunyun

Yang, Rui ^{[1
]}

Song, Jie ^{[1
]}

Wang, Siyao ^{[1
]}

Wang, Chunli ^{[1
]}

Ma, Ze ^{[1
]}

Li, Jiapeng ^{[1
]}

Feng, Tianqi ^{[1
]}

Hao, Linlin ^{[1
,4
]}

Li, Changhong ^{[2
]}

机构：

[1] Jilin Univ, Coll Anim Sci, Jilin, Peoples R China

[2] Baicheng Normal Univ, Coll Life Sci, Jilin, Peoples R China

[3] Tecon Biol Co Ltd, Urumqi, Peoples R China

[4] Jilin Univ, Coll Anim Sci, 5333 Xian Rd, Changchun 130062, Jilin, Peoples R China

来源：

VETERINARSKI ARHIV | 2022年 / 92卷 / 05期

关键词：

Chinese water buffalo (Bubalus bubalis); ARID4A; sperm quality; single-nucleotide polymorphisms; GENOME-WIDE ASSOCIATION; EXPRESSION; FERTILITY; MUTATIONS; SWAMP; IDENTIFICATION; TRAITS; ARID4A; BOAR;

D O I：

10.24099/vet.arhiv.1736

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

ARID4A (AT-rich interaction domain 4A) is closely related to animal sperm quality traits. In the present study, the association between ARID4A gene polymorphisms of Chinese water buffalo (Bubalus bubalis) with sperm quality traits was examined, including ejaculate volume, sperm concentration, post-thaw sperm motility, and sperm abnormality of buffalo semen. Seven single-nucleotide polymorphisms (SNPs) ofARID4A gene were detected in 156 Chinese water buffaloes by Sanger sequencing and identifying overlap peaks. Among the SNPs, six were associated with at least one sperm quality trait. In brief, g.21192G>C, g.21285C>G, and g.21364A>G could be used as potential markers for selecting semen with low sperm abnormality, high ejaculate volume, sperm concentration, and sperm motility. Furthermore, 10 haplotypes (H1:-CTCGG, H2: GTGGCA, H3: GCGGCA, H4: GCTGCA, H5: GCTCGA, H6: GTGGGG, H7: GCTCCG, H8:-CGGGA, H9: GCGGCG, and H10: GTTGCA) were formed by the six SNPs through linkage disequilibrium analysis, and then 14 different combined haplotypes were collected. Correlation analysis showed that the combined H1H2 haplotype had the highest genotype frequency. Notably, the combined H1H2 haplotype had low sperm concentration, low sperm motility, and high sperm abnormality. The combined H2H3 haplotype could be used as a potential molecular marker for selecting semen with high sperm motility. In general, we illustrated a significant correlation between SNPs in ARID4A and sperm quality traits of Chinese water buffalo, which may be useful in the marker-assisted selection of buffalo breeding. This study was the first to analyze the genetic polymorphisms ofARID4A and association with sperm qualities of Chinese buffalo.

引用

页码：577 / 590

页数：14

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