DETECTION OF VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS FROM A FIELD OUTBREAK IN CENTRAL INDIA

被引：2

作者：

Singh, Azad ^{[1
]}

Bedekar, Megha Kadam ^{[1
]}

Sharma, Rakesh ^{[2
]}

Sarkhel, Bikash Chandra ^{[1
]}

Singh, Sanjeev ^{[1
]}

Jain, Sudhir Kumar ^{[1
]}

机构：

[1] MPPCVV, Anim Biotechnol Ctr, Jabalpur 482004, MP, India

[2] Phoenix Diagnost Lab, Jabalpur, India

来源：

ACTA VETERINARIA HUNGARICA | 2012年 / 60卷 / 01期

关键词：

Infectious bursal disease; IBD virus; VP2; gene; RT-PCR; sequencing of VP2 gene; phylogenetic analysis; SEQUENCE-ANALYSIS; MOLECULAR CHARACTERIZATION; CHICKENS; SEGMENTS; STRAINS; GENOME;

D O I：

10.1556/AVet.2012.014

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In order to detect infectious bursal disease virus (IBDV), bursal tissue was collected from 10 IBD-suspected birds from a 30-day-old, IBDV-vaccinated commercial broiler chicken flock of 2000 birds exhibiting clinical signs suggestive of infectious bursal disease (IBD). The presence of IBDV was confirmed by partial amplification of the VP2 gene by reverse transcription and polymerase chain reaction. Isolates were identified as very virulent strains of IBDV (vvIBDV) by nucleotide sequence analysis. The comparison of the VP2 nucleotide sequences among the isolates revealed the presence of single-nucleotide polymorphisms in the VP2 gene of IBDV in the same flock. The comparative analysis indicated that these viruses were genetically close to the vvIBDVs previously detected in India. Our analysis provided information about the existence of vvIBDV in Central India.

引用

页码：165 / 174

页数：10

共 12 条

[1] Detection of infectious bursal disease virus in different lymphoid organs by single-step reverse transcription polymerase chain reaction and microplate hybridization assay
Barlic-Maganja, D
Zorman-Rojs, O
Grom, J
[J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2002, 14 (03) : 243 - 246
[2] VP2 SEQUENCES OF RECENT EUROPEAN VERY VIRULENT ISOLATES OF INFECTIOUS BURSAL DISEASE VIRUS ARE CLOSELY-RELATED TO EACH OTHER BUT ARE DISTINCT FROM THOSE OF CLASSICAL STRAINS
BROWN, MD
GREEN, P
SKINNER, MA
[J]. JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 675 - 680
[3] SEQUENCE-ANALYSIS AND EXPRESSION OF THE HOST-PROTECTIVE IMMUNOGEN-VP2 OF A VARIANT STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS WHICH CAN CIRCUMVENT VACCINATION WITH STANDARD TYPE-I STRAINS
HEINE, HG
HARITOU, M
FAILLA, P
FAHEY, K
AZAD, A
[J]. JOURNAL OF GENERAL VIROLOGY, 1991, 72 : 1835 - 1843
[4] Molecular characterization of Brazilian infectious bursal disease viruses
Ikuta, N
El-Attrache, J
Villegas, P
García, M
Lunge, VR
Fonseca, ASK
Oliveira, C
Marques, EK
[J]. AVIAN DISEASES, 2001, 45 (02) : 297 - 306
[5] Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity
Jackwood, D. J.
Sreedevi, B.
LeFever, L. J.
Sommer-Wagner, S. E.
[J]. VIROLOGY, 2008, 377 (01) : 110 - 116
[6] Genetic characteristics of infectious bursal disease viruses from four continents
Jackwood, Daral J.
Sommer-Wagner, Susan
[J]. VIROLOGY, 2007, 365 (02) : 369 - 375
[7] Sequence Analysis of Both Genome Segments of Three Croatian Infectious Bursal Disease Field Viruses
Lojkic, I.
Bidin, Z.
Pokric, B.
[J]. AVIAN DISEASES, 2008, 52 (03) : 513 - 519
[8] Molecular characterization of Indian isolates of infectious bursal disease virus from broiler chickens
Mittal, Dinesh
Jindal, Naresh
Gupta, Shiv L.
Kataria, Ranjit S.
Singh, Kamaljit
Tiwari, Ashok K.
[J]. DNA SEQUENCE, 2006, 17 (06): : 431 - 439
[9] MULLER H, 1979, J VIROL, V31, P584
[10] Nagarajan MM, 1997, CAN J VET RES, V61, P81

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