Real-time PCR detection of Mycoplasma felis in domestic cats suffering from chronic conjunctivitis (Poland)

被引:3
作者
Ploneczka-Janeczko, K. [1 ]
Kielbowicz, Z. [2 ]
Bania, J. [3 ]
Bednarek, K. [1 ]
机构
[1] Wroclaw Univ Environm & Life Sci, Clin Birds & Exot Anim, Dept Epizootiol, Wroclaw, Poland
[2] Wroclaw Univ Environm & Life Sci, Dept & Clin Vet Surg, Wroclaw, Poland
[3] Wroclaw Univ Environm & Life Sci, Fac Vet Med, Dept Food Hyg & Consumer Hlth Protect, Wroclaw, Poland
来源
POLISH JOURNAL OF VETERINARY SCIENCES | 2011年 / 14卷 / 04期
关键词
Mycoplasma felis; domestic cats; chronic conjunctivitis; real-time PCR; POLYMERASE-CHAIN-REACTION; CHLAMYDOPHILA-FELIS; DISEASE;
D O I
10.2478/v10181-011-0103-y
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Real-time PCR directed to intergenic spacer (IGS) noncoding region between 16S and 23S rRNA genes was used for species specific detection of Mycoplasma felis in conjunctival scrapings. Samples were collected from 57 cats suffering from chronic conjunctivitis in 2008-2010 (Wroclaw, Poland). Samples from 36 cats (63.2%) were shown to be positive for Mycoplasma fells. Our research gives a first insight in the occurrence of Mycoplasma fells among domestic cats in Poland suggesting that this pathogen may constitute an underestimated cause of chronic conjunctivitis.
引用
收藏
页码:679 / 681
页数:3
相关论文
共 7 条
[1]   Development of a polymerase chain reaction for the detection of Mycoplasma felis in domestic cats [J].
Chalker, VJ ;
Owen, WMA ;
Paterson, CJI ;
Brownlie, J .
VETERINARY MICROBIOLOGY, 2004, 100 (1-2) :77-82
[2]   Detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease [J].
Hartmann, Anja D. ;
Hawley, Jennifer ;
Werckenthin, Christiane ;
Lappin, Michael R. ;
Hartmann, Katrin .
JOURNAL OF FELINE MEDICINE AND SURGERY, 2010, 12 (10) :775-782
[3]   Prevalence of feline herpesvirus 1, Chlamydophila felis, and Mycoplasma spp DNA in conjunctival cells collected from cats with and without conjunctivitis [J].
Low, Heather C. ;
Powell, Cynthia C. ;
Veir, Julia K. ;
Hawley, Jennifer R. ;
Lappin, Michael R. .
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2007, 68 (06) :643-648
[4]  
Maggs DJ., 2008, SLATTERS FUNDAMENTAL, VFourth, P135
[5]  
Sandmeyer LS, 2010, CAN VET J, V51, P629
[6]   Evaluation of different sampling methods and results of real-time PCR for detection of feline herpes virus-1, Chlamydophila felis and Mycoplasma felis in cats [J].
Sjodahl-Essen, Titti ;
Tidholm, Anna ;
Thoren, Peter ;
Persson-Wadman, Annie ;
Bolske, Goran ;
Aspan, Anna ;
Berndtsson, Louise Treiberg .
VETERINARY OPHTHALMOLOGY, 2008, 11 (06) :375-380
[7]   Feline chlamydiosis [J].
Sykes, JE .
CLINICAL TECHNIQUES IN SMALL ANIMAL PRACTICE, 2005, 20 (02) :129-134