Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template

被引：50

作者：

El-Sagheer, Afaf H. ^{[1
,2
]}

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Suez Canal Univ, Fac Petr & Min Engn, Chem Branch, Dept Sci & Math, Suez 43721, Egypt

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 44期

关键词：

POLYMERASE;

D O I：

10.1039/c1cc14316f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.

引用

页码：12057 / 12058

页数：2

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