Molecular evidence for SARS-CoV-2 in samples collected from patients with morbilliform eruptions since late 2019 in Lombardy, northern Italy

被引：12

作者：

Amendola, Antonella ^{[1
,2
]}

Canuti, Marta ^{[1
]}

Bianchi, Silvia ^{[1
,2
]}

Kumar, Sudhir ^{[3
,4
,5
]}

Fappani, Clara ^{[1
,2
]}

Gori, Maria ^{[1
,2
]}

Colzani, Daniela ^{[1
,2
]}

Pond, Sergei L. Kosakovsky ^{[3
,4
]}

Miura, Sayaka ^{[3
,4
]}

Baggieri, Melissa ^{[6
]}

Marchi, Antonella ^{[6
]}

Borghi, Elisa ^{[1
,2
]}

Zuccotti, Gianvincenzo ^{[7
,8
]}

Raviglione, Mario C. ^{[9
]}

Magurano, Fabio ^{[6
]}

Tanzi, Elisabetta ^{[1
,2
,10
]}

机构：

[1] Univ Milan, Dept Hlth Sci, I-20142 Milan, Italy

[2] Univ Milan, Coordinated Res Ctr EpiSoMI, I-20133 Milan, Italy

[3] Temple Univ, Inst Genom & Evolutionary Med, Philadelphia, PA 19122 USA

[4] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA

[5] King Abdulaziz Univ, Ctr Excellence Genome Med & Res, Jeddah 22252, Saudi Arabia

[6] Ist Super Sanita, Dept Infect Dis, I-00161 Rome, Italy

[7] Univ Milan, Children Hosp V Buzzi, Dept Paediat, I-20154 Milan, Italy

[8] Univ Milan, Romeo & Enrica Invernizzi Pediat Res Ctr, I-20154 Milan, Italy

[9] Univ Milan, Ctr Multidisciplinary Res Hlth Sci, I-20122 Milan, Italy

[10] Via Pascal 36, I-20133 Milan, Italy

来源：

ENVIRONMENTAL RESEARCH | 2022年 / 215卷

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

SARS-CoV-2; COVID-19; Pandemic; Emergence; Measles; Rash; IMMUNITY; MILAN;

D O I：

10.1016/j.envres.2022.113979

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

As a reference laboratory for measles and rubella surveillance in Lombardy, we evaluated the association between SARS-CoV-2 infection and measles-like syndromes, providing preliminary evidence for undetected early circulation of SARS-CoV-2. Overall, 435 samples from 156 cases were investigated. RNA from oropharyngeal swabs (N = 148) and urine (N = 141) was screened with four hemi-nested PCRs and molecular evidence for SARS-CoV-2 infection was found in 13 subjects. Two of the positive patients were from the pandemic period (2/12, 16.7%, March 2020-March 2021) and 11 were from the pre-pandemic period (11/44, 25%, August 2019-February 2020). Sera (N = 146) were tested for anti-SARS-CoV-2 IgG, IgM, and IgA antibodies. Five of the RNA-positive individuals also had detectable anti-SARS-CoV-2 antibodies. No strong evidence of infection was found in samples collected between August 2018 and July 2019 from 100 patients. The earliest sample with evidence of SARS-CoV-2 RNA was from September 12, 2019, and the positive patient was also positive for anti-SARS-CoV-2 antibodies (IgG and IgM). Mutations typical of B.1 strains previously reported to have emerged in January 2020 (C3037T, C14408T, and A23403G), were identified in samples collected as early as October 2019 in Lombardy. One of these mutations (C14408T) was also identified among sequences downloaded from public databases that were obtained by others from samples collected in Brazil in November 2019. We conclude that a SARS-CoV-2 progenitor capable of producing a measles-like syndrome may have emerged in late June-late July 2019 and that viruses with mutations characterizing B.1 strain may have been spreading globally before the first Wuhan outbreak. Our findings should be complemented by high-throughput sequencing to obtain additional sequence information. We highlight the importance of retrospective surveillance studies in understanding the early dynamics of COVID-19 spread and we encourage other groups to perform retrospective investigations to seek confirmatory proofs of early SARS-CoV-2 circulation.

引用

页数：9