Label-Free Detection of Single-Base Mismatches in DNA by Surface-Enhanced Raman Spectroscopy

被引：166

作者：

Papadopoulou, Evanthia ^{[1
]}

Bell, Steven E. J. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Chem & Chem Engn, Innovat Mol Mat Grp, Belfast BT9 5AG, Antrim, North Ireland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 39期

关键词：

DNA; nanomaterials; nucleotides; RNA; SERS; NUCLEOTIDE POLYMORPHISMS; NANOPARTICLES; COMPLEXES;

D O I：

10.1002/anie.201102776

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Singles only: DNA sequences can be induced to spontaneously adsorb to the surfaces of Ag colloids through their nucleotide side chains (see picture). The SERS spectra of these nonspecifically bound strands are sufficiently reproducible that they can be used to identify single-base mismatches in short (25-mer and 23-mer) strands. Subtracting the spectra of different DNA sequences results in difference spectra that contain features corresponding to the exchanged nucleotides. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9058 / 9061

页数：4

共 14 条

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Bailo E., 2008, ANGEW CHEM, V120, p1682

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