A comprehensive study of the harmful effects of ZnO nanoparticles using Drosophila melanogaster as an in vivo model

被引：57

作者：

Alaraby, Mohamed ^{[1
,2
]}

Annangi, Balasubramanyam ^{[1
]}

Hernandez, Alba ^{[1
,3
]}

Creus, Amadeu ^{[1
,3
]}

Marcos, Ricard ^{[1
,3
]}

机构：

[1] Univ Autonoma Barcelona, Fac Biociencies, Dept Genet & Microbiol, Grp Mutagenesi, E-08193 Barcelona, Spain

[2] Sohag Univ, Dept Zool, Fac Sci, Sohag, Egypt

[3] ISCIII, CIBER Epidemiol & Salud Publ, Madrid, Spain

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2015年 / 296卷

关键词：

Drosophila melanogaster; ZnO nanoparticles; Genotoxicity; Comet assay; Wing-spot assay; ZINC-OXIDE NANOPARTICLES; OXIDATIVE STRESS; GENOTOXICITY; CYTOTOXICITY; APOPTOSIS; TOXICITY; CELLS; EXPRESSION; SUNSCREENS; EARTHWORM;

D O I：

10.1016/j.jhazmat.2015.04.053

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study planned to determine the range of biological effects associated with ZnO-NP exposure using Drosophila melanogaster as an in vivo model. In addition, ZnCl2 was used to determine the potential role of Zn ions alone. Toxicity, internalization through the intestinal barrier, gene expression changes, ROS production, and genotoxicity were the end-points evaluated. No toxicity or oxidative stress induction was observed in D. melanogaster larvae, whether using ZnO-NPs or ZnCl2. Internalization of ZnO-NPs through the intestinal barrier was observed. No significant changes in the frequency of mutant clones (wing-spot test) or percentage of DNA in tail (comet assay) were observed although significant changes in Hsp70 and p53 gene expression were detected. Our study shows that ZnO-NPs do not induce toxicity or genotoxicity in D. melanogaster, although uptake occurs and altered gene expression is observed. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：166 / 174

页数：9

共 46 条

[1] ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: role of oxidative stress [J].