Curvature factor and membrane solubilization, with particular reference to membrane rafts

被引:11
|
作者
Mrowczynska, Lucyna [2 ]
Salzer, Ulrich [3 ]
Iglic, Ales [4 ]
Hagerstrand, Henry [1 ]
机构
[1] Abo Akad Univ, Dept Biosci, FIN-20520 Turku, Finland
[2] Adam Mickiewicz Univ, Dept Cell Biol, PL-61614 Poznan, Poland
[3] Med Univ Vienna, Max F Perutz Labs, A-1030 Vienna, Austria
[4] Fac Elect Engn, Biophys Lab, SI-1000 Ljubljana, Slovenia
关键词
detergent-resistant membrane; membrane curvature; raft; solubilization; Triton X-100; DETERGENT-RESISTANT MEMBRANES; ERYTHROCYTES FOLLOWING TREATMENT; LIPID RAFTS; PLASMA-MEMBRANE; CELL-MEMBRANES; TRITON X-100; PHYSIOLOGICAL TEMPERATURE; SHAPE TRANSFORMATIONS; BIOLOGICAL-MEMBRANES; PROTEIN-COMPOSITION;
D O I
10.1042/CBI20100786
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The composition of membrane rafts (cholesterol/sphingolipid-rich domains) cannot be fully deduced from the analysis of a detergent-resistant membrane fraction after solubilization in Triton X-100 at 4 degrees C. It is hypothesized that the membrane curvature-dependent lateral distribution of membrane components affects their solubilization. The stomatocytogenic, Triton X-100, cannot effectively solubilize membrane components, especially with regard to the outward membrane curvature.
引用
收藏
页码:991 / 995
页数:5
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