One-pot biosynthesis of silver nanoparticles using green tea plant extract/rosemary oil and investigation of their antibacterial activity

Silver nanoparticles is successfully biosynthesized in a one-pot procedure by using a mixture of green tea extract (GTE) and Rosemary oil (RO). Different relative ratios of GTE, RO and AgNO3 in reaction mixture has affected the sizes and concentration of produced nanoparticles. Increasing the relative concentrations of GTE/RO resulted to the increase in size of obtained SNPs, and optimal conditions is observed with relative ratio of RO(1 mL), GTE(0.135 mg) and AgNO3(15.3 mg) producing 9 nm nanoparticles. By varying ratio of GTE(g)/AgNO3(mol) from 0.4 to 2.8, the optimum results and smallest nanoparticles has observed at ratio of 1.5. Antibacterial activity of the products is evaluated against various bacteria strains. The maximum inhibition zone diameter is observed for optimal sample against S. dysenteriae, S. epidermidis and S. pyogenes strains. The optimal sample capped with PVA nanofibers (94 nm) has exhibited a sensibly better antibacterial performance as compared to bare nanoparticles, with almost 15% larger exhibition zones than other samples.