New Methods for Detection of Campylobacters in Stool Samples in Comparison to Culture

被引：102

作者：

Bessede, Emilie ^{[2
,3
]}

Delcamp, Adline ^{[2
]}

Sifre, Elodie ^{[2
]}

Buissonniere, Alice ^{[2
]}

Megraud, Francis ^{[1
,2
,3
]}

机构：

[1] Hop Pellegrin, CHU Bordeaux, CNR Campylobacters, Bacteriol Lab, F-33076 Bordeaux, France

[2] Ctr Natl Reference Campylobacters & Helicobacters, F-33076 Bordeaux, France

[3] INSERM, U853, F-33076 Bordeaux, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期

关键词：

MICROPLATE ASSAY; JEJUNI; INFECTIONS; PCR;

D O I：

10.1128/JCM.01489-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Campylobacter species, especially Campylobacter jejuni and Campylobacter coli, are a major cause of human bacterial enteritis. Current detection in stools is done essentially by culture on selective and nonselective media with filtration. These methods were compared to 2 molecular biology methods, an in-house real-time PCR and a multiplex PCR named Seeplex Diarrhea ACE Detection, and 3 immunoenzymatic methods, Premier Campy, RidaScreen Campylobacter, and ImmunoCard Stat!Campy. Out of 242 stool specimens tested, 23 (9.5%) fulfilled the positivity criteria, i.e., they were positive by one or both culture methods or, in case of a negative culture, by a positive molecular method and a positive immunoenzymatic method. The striking feature of this study is the low sensitivity of culture, in the range of 60%, in contrast to immunoenzymatic and molecular tests.

引用

页码：941 / 944

页数：4