Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase

被引：71

作者：

Xuan Canh Nguyen ^{[1
]}

Kim, Sun Ho ^{[2
,3
]}

Lee, Kyunghee ^{[1
]}

Kim, Kyung Eun ^{[1
]}

Liu, Xiao-Min ^{[1
]}

Hay Ju Han ^{[1
]}

My Hanh Thi Hoang ^{[1
]}

Lee, Shin-Woo ^{[4
]}

Hong, Jong Chan ^{[1
,2
]}

Moon, Yong-Hwan ^{[3
]}

Chung, Woo Sik ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci, Program BK21, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Jinju 660701, South Korea

[3] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[4] Gyeongnam Natl Univ Sci & Technol, Div Agron & Med Resources, Jinju, South Korea

来源：

PLANT CELL REPORTS | 2012年 / 31卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Arabidopsis; C2H2-type zinc finger; MPK; Phosphorylation; Substrate; ZAT10; ACTIVATED PROTEIN-KINASE; STRESS; PLANTS; PHOSPHORYLATION; THALIANA; ATMPK6; TOLERANCE; RESPONSES; NETWORKS; CASCADES;

D O I：

10.1007/s00299-011-1192-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. MPKs can directly modulate gene expression by the phosphorylation of transcription factors. However, only a few target substrates of MPKs have been isolated. Here, we identified a C2H2-type zinc finger transcription factor from Arabidopsis, ZAT10, as a substrate of MPKs. Using in vitro and in vivo protein-protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and MPK6. ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. Furthermore, ZAT10 was also phosphorylated by native MPK3 and MPK6 prepared from Arabidopsis plants in an in-gel kinase assay. Mass spectrometry analysis of phosphopeptides was used to determine two MPK phosphorylation sites in ZAT10. These sites were verified by site-directed mutagenesis and in vitro kinase assays.

引用

页码：737 / 745

页数：9

共 33 条

[31] Plant mitogen-activated protein kinase signaling cascades [J].