Plasmin is involved in inflammation via protease-activated receptor-1 activation in human dental pulp

被引：34
作者：
Kamio, Naoto [2 ]
Hashizume, Hideki [2 ,4 ]
Nakao, Sumi [3 ,4 ]
Matsushima, Kiyoshi [2 ,4 ]
Sugiya, Hiroshi [1 ,4 ]
机构：
[1] Nihon Univ, Sch Dent Matsudo, Dept Physiol, Chiba 2718587, Japan
[2] Nihon Univ, Sch Dent Matsudo, Dept Endodont, Chiba 2718587, Japan
[3] Nihon Univ, Sch Dent Matsudo, Dept Oral Mol Pharmacol, Chiba 2718587, Japan
[4] Nihon Univ, Sch Dent Matsudo, Res Inst Oral Sci, Chiba 2718587, Japan
来源：
BIOCHEMICAL PHARMACOLOGY | 2008年 / 75卷 / 10期
基金：
日本学术振兴会;
关键词：
plasmin; protease-activated receptor-1; Ca2+ mobilization; inflammation; dental pulp;
D O I：
10.1016/j.bcp.2008.02.018
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Plasmin is a proteolytic enzyme produced from plasminogen by plasminogen activators. We investigated the function of plasmin in human dental pulp fibroblast-like cells. Plasmin induced an increase in the intracellular Ca2+ concentration ([Ca2+](i)) in a concentration-dependent manner. Expression of mRNA for protease-activated receptor-1 (PAR-1) was detected, and the PAR-1 activating peptide SFLLRN induced an increase in [Ca2+](i) in the cells. The plasmin-induced increase in [Ca2+](i) was inhibited in the presence of the PAR-1 antagonist SCH79797. Plasmin stimulated the expression of interleukin-8 (IL-8) mRNA and prostaglandin E-2 release, which are involved in inflammation. These effects of plasmin on expression of IL-8 mRNA and prostaglandin E-2 release were inhibited in the presence of the PAR-1 antagonist SCH79797. These results suggest that plasmin activates PAR-1 and is involved in inflammation in human dental pulp. (C) 2008 Elsevier Inc. All rights reserved
引用
收藏
页码：1974 / 1980
页数：7
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