Recovery of Chitin from Antarctic Krill (Euphausia superba) Shell Waste by Microbial Deproteinization and Demineralization

被引：15

作者：

Chen, Xuejiao ^{[1
]}

Jiang, Qixing ^{[1
]}

Xu, Yanshun ^{[1
]}

Xia, Wenshui ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, 1800 Lihu Ave, Wuxi, Jiangsu, Peoples R China

来源：

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY | 2017年 / 26卷 / 10期

基金：

国家高技术研究发展计划(863计划);

关键词：

Krill shell waste; Bacillus subtilis; Lactobacillus plantarum; deproteinization (DP); demineralization (DM); chitin; BACILLUS-SUBTILIS FERMENTATION; SHRIMP WASTES; CHITOSAN; PURIFICATION; BIOWASTE; PROTEASE;

D O I：

10.1080/10498850.2015.1094686

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A two-stage fermentation process of deproteinization and demineralization was used to purify chitin from Antarctic krill shell waste. Two bacterial cultures were isolated and tested for the degradation of krill shell waste. According to the morphological examination, physiological tests, and applied molecular techniques conducted, isolates were identified as Bacillus subtilis and Lactobacillus plantarum. At a temperature of 37 degrees C, both strains were cultivated separately in flasks containing krill shell waste. At determined periods of time, deproteinization and demineralization of residuals were measured. For deproteinization tests, the liquid phase fermentation of krill shell waste showed protein removal of 88.9% after 60 h. For demineralization tests, the liquid phase fermentation of krill shell waste showed calcium carbonate removal of 84.6% after 72 h. Fourier transform infrared spectroscopy (FTIR) analysis of chitin prepared by the process was carried out and compared with spectra of samples treated by strong acids and bases.

引用

页码：1210 / 1220

页数：11

共 33 条

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